Abstract The increasing antibiotic application in agriculture has raised a potential risk to human health worldwide. Although high concentrations of antibiotics are lethal for plants without a resistant gene, it is not clear what detoxifying processes act in plants under low antibiotic concentration. Arabidopsis thaliana was selected to characterize the effects of kanamycin antibiotic toxicity on seedling growth in MS plates and pots, respectively. We investigated only control plants treated with kanamycin; but without comparison with transgenic ones, since we had no positive control transgenic plants. Gene expression profiles from plant material in pots were evaluated by customized oligonucleotide microarray. Morphological investigation indicated that kanamycin concentration is negatively correlated with A. thaliana seedling growth. Gene expression profile analysis detected that 542 and 116 genes were up- and down-regulated over five-fold in leaf tissue under the influence of kanamycin. The glutathione S-transferase and peroxidase gene families were involved in antioxidant defense mechanisms by preventing the accumulation of toxic products of reactive oxygen species (ROS). An exceeding amount of ROS after antibiotic-induced oxidative burst stimulated several over-presented processes for the balance between ROS and antioxidants. The significant increase in detoxification enzymes may indicate that efficient antioxidant machinery is the main method to ensure survival.

中文翻译：

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卡那霉素对拟南芥幼苗生长胁迫的解毒过程

摘要 抗生素在农业中的应用日益增加，给全世界人类健康带来了潜在风险。虽然高浓度的抗生素对没有抗性基因的植物是致命的，但尚不清楚在低抗生素浓度下植物中的解毒过程是什么。选择拟南芥来分别表征卡那霉素抗生素毒性对 MS 平板和盆中幼苗生长的影响。我们只调查了用卡那霉素处理的对照植物；但没有与转基因植物进行比较，因为我们没有阳性对照转基因植物。通过定制的寡核苷酸微阵列评估盆栽植物材料的基因表达谱。形态学研究表明，卡那霉素浓度与拟南芥幼苗生长呈负相关。基因表达谱分析检测到，在卡那霉素作用下，叶组织中有542个和116个基因上调和下调超过5倍。谷胱甘肽 S 转移酶和过氧化物酶基因家族通过防止活性氧 (ROS) 有毒产物的积累参与抗氧化防御机制。抗生素诱导的氧化爆发后过量的 ROS 刺激了几种过度呈现的过程，以实现 ROS 和抗氧化剂之间的平衡。解毒酶的显着增加可能表明有效的抗氧化机制是确保生存的主要方法。谷胱甘肽 S 转移酶和过氧化物酶基因家族通过防止活性氧 (ROS) 有毒产物的积累参与抗氧化防御机制。抗生素诱导的氧化爆发后过量的 ROS 刺激了几种过度呈现的过程，以实现 ROS 和抗氧化剂之间的平衡。解毒酶的显着增加可能表明有效的抗氧化机制是确保生存的主要方法。谷胱甘肽 S 转移酶和过氧化物酶基因家族通过防止活性氧 (ROS) 有毒产物的积累参与抗氧化防御机制。抗生素诱导的氧化爆发后过量的 ROS 刺激了几种过度呈现的过程，以实现 ROS 和抗氧化剂之间的平衡。解毒酶的显着增加可能表明有效的抗氧化机制是确保生存的主要方法。