Arrayan and peumo fruits are commonly used in the traditional medicine of Chile. In this study, the concentration of the extracts halving the bacterial viability and biofilms formation and disruption of the drug-sensitive and drug-resistant strains of Staphylococcus aureus and Pseudomonas aeruginosa was determined. The chemical composition of extracts was analyzed by high-resolution liquid chromatography coupled with mass spectrometry (U-HPLC/MS). The arrayan extract (Inhibitory concentration IC₅₀ 0.35 ± 0.01 mg/mL) was more effective than peumo extract (IC₅₀ 0.53 ± 0.02 mg/mL) in the inhibition of S. aureus planktonic cells. Similarly, the arrayan extract was more effective in inhibiting the adhesion (S. aureus IC₅₀ 0.23 ± 0.02 mg/mL, P. aeruginosa IC₅₀ 0.29 ± 0.02 mg/mL) than peumo extracts (S. aureus IC₅₀ 0.47 ± 0.03 mg/mL, P. aeruginosa IC₅₀ 0.35 ± 0.01 mg/mL). Both extracts inhibited quorum sensing in a concentration-dependent manner, and the most significant was the autoinducer-2 type communication inhibition by arrayan extract. Both extracts also disrupted preformed biofilm of P. aeruginosa (arrayan IC₅₀ 0.56 ± 0.04 mg/mL, peumo IC₅₀ 0.59 ± 0.04 mg/mL). However, neither arrayan nor peumo extracts disrupted S. aureus mature biofilm. U-HPLC/MS showed that both fruit extracts mainly possessed quercetin compounds; the peumo fruit extract also contained phenolic acids and phenylpropanoids. Our results suggested that both extracts could be used as natural antimicrobials for some skin and nosocomial infections.

中文翻译：

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智利两种原生水果提取物的抗菌活性：Arrayan（Luma apiculata）和Peumo（Cryptocarya alba）。

Arrayan和peumo水果是智利传统医学中常用的水果。在这项研究中，确定了将细菌活力和生物膜形成减半的提取物浓度，以及对金黄色葡萄球菌和铜绿假单胞菌的药物敏感和耐药菌株的破坏。提取物的化学成分通过高分辨率液相色谱-质谱联用（U-HPLC / MS）进行分析。Arrayan提取物（抑制浓度IC ₅₀ 0.35±0.01 mg / mL）在抑制金黄色葡萄球菌浮游细胞方面比peumo提取物（IC ₅₀ 0.53±0.02 mg / mL）更有效。同样，array聚糖提取物在抑制粘连方面更有效（金黄色葡萄球菌IC ₅₀ 0.23±0.02 mg / mL，铜绿假单胞菌IC ₅₀ 0.29±0.02 mg / mL）比脓毒提取物（金黄色葡萄球菌IC ₅₀ 0.47±0.03 mg / mL，铜绿假单胞菌IC ₅₀ 0.35±0.01 mg / mL）毫升）。两种提取物均以浓度依赖的方式抑制群体感应，而最显着的是arrayan提取物对autoinducer-2型通讯的抑制作用。两种提取物还破坏了铜绿假单胞菌的预先形成的生物膜（arrayan IC ₅₀ 0.56±0.04 mg / mL，pemomo IC ₅₀ 0.59±0.04 mg / mL）。然而，既没有arrayan也没有peumo提取物破坏金黄色葡萄球菌成熟的生物膜。U-HPLC / MS分析表明，两种水果提取物均主要具有槲皮素化合物。peumo水果提取物还含有酚酸和苯丙烷。我们的结果表明，两种提取物均可用作某些皮肤和医院感染的天然抗菌剂。