Learning more about the biodiversity and composition of arbuscular mycorrhizal fungi (AMF) under alternative agricultural management scenarios may be important to the sustainable intensification of switchgrass grown as a bioenergy crop. Using PacBio single-molecule sequencing and taxonomic resolution to the level of amplicon sequence variant (ASV), we assessed the effects of nitrogen amendment on AMF associating with switchgrass and explored relationships between AMF and switchgrass yield across three sites of various productivities in Wisconsin. Nitrogen amendment had little effect on AMF diversity metrics or community composition. While AMF ASV diversity was not correlated with switchgrass yield, AMF family richness and switchgrass yield had a strong, positive relationship at one of our three sites. Each of our sites was dominated by unique ASVs of the species Paraglomus brasilianum, indicating regional segregation of AMF at the intraspecific level. Our molecular biodiversity survey identified putative core members of the switchgrass microbiome, as well as novel clades of AMF, especially in the order Paraglomerales and the genus Nanoglomus. Furthermore, our phylogenies unite the cosmopolitan, soil-inhabiting clade deemed GS24 with Pervetustaceae, an enigmatic family prevalent in stressful environments. Future studies should isolate and characterize the novel genetic diversity found in switchgrass agroecosystems and explore the potential yield benefits of AMF richness.

中文翻译：

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生物能源收获的柳枝Soil土壤中丛枝菌根真菌的群落结构。

在替代性农业管理方案下，更多地了解丛枝菌根真菌（AMF）的生物多样性和组成可能对作为生物能源作物种植的柳枝switch的可持续集约化很重要。使用PacBio单分子测序和分类学解析到扩增子序列变异体（ASV）的水平，我们评估了氮改良对柳枝switch与AMF的影响，并探索了威斯康星州三个生产力不同地点的AMF和柳枝yield产量之间的关系。氮素改良对AMF多样性指标或社区组成影响不大。虽然AMF ASV多样性与柳枝switch产量不相关，但AMF家族的丰富程度和柳枝yield产量在我们三个地点之一之间具有很强的正相关关系。Brasiliarum brasilianum，表明种内水平的AMF区域隔离。我们的分子生物多样性调查确定了柳枝micro微生物组的假定核心成员，以及新的AMF进化枝，特别是顺伞目目和纳米球目属。此外，我们的系统发育学将被认为是GS24的国际化，土壤栖息的进化枝与Pervetustaceae结合在一起，Pervetustaceae是一个在压力环境中普遍存在的神秘家庭。未来的研究应隔离和表征柳枝a农业生态系统中发现的新遗传多样性，并探索AMF丰富度的潜在产量优势。