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Cenostigma tocantinum, a new host of Colletotrichum siamense
Forest Pathology ( IF 1.3 ) Pub Date : 2020-07-25 , DOI: 10.1111/efp.12623
Ana Francisca Tibúrcia A. Ferreira e Ferreira 1 , Blenda N. S. Silva 1 , Adolfo J. Mota 2 , Jânia Lília S. B. Lima 1
Affiliation  

Trees of Cenostigma tocantinum showing brown leaf spots surrounded by chlorotic areas were observed at the campus of the Federal University of Amazonas, Brazil, in 2018. Necrotic lesions were covered by orange masses of hyaline cylindrical conidia with rounded ends, typical of the genus Colletotrichum. Three monoconidial isolates were subject of DNA extraction followed by PCR amplification and sequencing of the ribosomal internal transcribed spacer (ITS rDNA), beta‐tubulin 2 (TUB2) and glycerol‐3‐phosphate dehydrogenase (GAPDH) genes. Multilocus phylogenetic analyses and morphological characterization allowed the identification of the isolates as C. siamense, a member of the C. gloeosporioides species complex. The pathogenicity of C. siamense was confirmed, and Koch's postulates completed. This is the first worldwide report of C. siamense causing leaf spots on Ce. tocantinum.

中文翻译:

Cenostigma tocantinum,暹罗炭疽菌的新宿主

在2018年的巴西亚马逊大学(University of Amazonas)校园内观察到了Cenostigma tocantinum的树,树上有褐色的斑点,周围有褪绿色的区域。坏死的病灶被橙色透明圆形分生孢子的圆锥形分生孢子覆盖,典型的是Colletotrichum属。对三个单孢子分离株进行DNA提取,然后对核糖体内部转录间隔区(ITS rDNA),β-微管蛋白2(TUB2)和甘油3-磷酸脱氢酶(GAPDH)基因进行PCR扩增和测序。多基因座系统发育分析和形态学表征使得可以将分离物鉴定为C. siamenseC。gloeosporioides种复合体的成员。的致病性C. siamense被确认,并且Koch的假设完成。这是暹罗梭菌Ce上引起叶斑的全球首份报告Tocantinum
更新日期:2020-07-25
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