A novel system for separation and purification of egg pigments from the nondiapause red-egg mutant Re-nd in the silkworm, Bombyx mori.,Archives of Insect Biochemistry and Physiology

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A novel system for separation and purification of egg pigments from the nondiapause red-egg mutant Re-nd in the silkworm, Bombyx mori.
Archives of Insect Biochemistry and Physiology ( IF 1.5 ) Pub Date : 2020-07-24 , DOI: 10.1002/arch.21728
Guanwang Shen _{1,

2} , Haiyan Zhang _{1,

2,

3} , Ying Lin _{1,

2} , Wei Long _{1,

2} , Ping Zhao _{1,

2}

Affiliation

Re‐nd, which was induced from the wild‐type C108 by the chemical mutagen N‐methane‐N‐methylnitrourea, is a nondiapause red‐egg mutant of silkworm Bombyx mori. The special significance of the Re‐nd mutant is that it is an independent dominant mutant. The aim of this study was to establish the type of pigment responsible for the red coloration in the Re‐nd mutant eggs in silkworm. We compared the eggs of Re‐nd mutants with those of the other B. mori egg color strains and confirmed that the Re‐nd mutant is the only strain with red color and red pigment granules in nondiapause, showing this mutant belongs to the pigmentation in the serosa. We speculated that the red substance, which contributed to the bright red pigmentation for nondiapause eggs of the Re‐nd mutant, could potentially be a novel pigment according to its solubility, optimum absorption peak, and oxidation–reduction reaction. Moreover, we have successfully constituted the system for enrichment, extraction, and purification of the red substance responsible for the Re‐nd mutant, providing a new method for the separation and purification of other known and unknown pigments or substances.

中文翻译：

从家蚕Bonbyx mori的非滞育性红蛋突变体Re-nd中分离和纯化卵色素的新型系统。

再次，将其与野生型C108通过化学诱变剂诱导的ñ -甲Ñ -methylnitrourea，是一个nondiapause红卵突变的家蚕家蚕。Rend突变体的特殊意义是它是一个独立的显性突变体。这项研究的目的是确定导致家蚕Rend突变卵中红色着色的色素类型。我们将Re-nd突变体的卵与其他桑蚕B.卵色菌株的卵进行了比较，并证实了Re-nd该突变体是唯一在非滞育期具有红色和红色颜料颗粒的菌株，表明该突变体属于浆膜中的色素沉着。我们推测，红色物质可为Re-nd突变体的非滞育卵提供鲜红色的色素，根据其溶解度，最佳吸收峰和氧化还原反应，它可能是一种新型色素。此外，我们已经成功地构建了负责Rend突变体的红色物质的富集，提取和纯化系统，为分离和纯化其他已知和未知的颜料或物质提供了一种新方法。

更新日期：2020-07-24

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