This study was designed to check whether complexation of royal jelly (RJ) proteins with green tea extract enriched with EGCG, would enhance the bioavailability on C2BBe1 cells. The total phenolic and EGCG of green tea extract (GTex) as well as the protein level of RJ were measured. The best entrapment efficiency (30.47%) was noted at a 10:4 ratio (RJ:EGCG of GTex) to confirm the maximum EGCG‐RJ complexation. Followed by in vitro studies to check the cytotoxicity, morphological changes, EGCG uptake, and TBARS (antioxidant) activity were evaluated on C2BBe1 cells. The EGCG‐RJ protein complex showed less toxicity without any morphological changes with better cellular EGCG uptake than GTex or GTex‐RJ mixture on CeBBe1 cells. Besides, the EGCG‐RJ protein complex display maximum TBARS suppressing activity to showcase better stability. This study infers that complexation of RJ proteins with EGCG (EGCG‐RJ protein complex) could significantly improve the bioavailability of EGCG.

中文翻译：

---

与蜂王浆蛋白复合后，提高了EGCG的生物利用度。

这项研究旨在检查蜂王浆（RJ）蛋白与富含EGCG的绿茶提取物的复合是否会增强C2BBe1细胞的生物利用度。测量了绿茶提取物（GTex）的总酚和EGCG以及RJ的蛋白质水平。以10：4的比例（GTex的RJ：EGCG）观察到最佳包封率（30.47％），以确认最大的EGCG-RJ络合物。随后进行了体外研究以检查C2BBe1细胞的细胞毒性，形态变化，EGCG摄取和TBARS（抗氧化剂）活性。与GTex或GTex‐RJ混合物相比，与CeBBe1细胞相比，EGCG‐RJ蛋白复合物的毒性较小，没有任何形态学变化，并且细胞EGCG摄取更好。此外，EGCG-RJ蛋白复合物显示出最大的TBARS抑制活性，显示出更好的稳定性。