Aerobically growing Escherichia coli generates superoxide flux into the periplasm via the oxidation of dihydromenaquinone and simultaneously carries out continuous transmembrane cycling of glutathione (GSH). Here we have shown that, under the conditions of a gradual decrease in dissolved oxygen (dO₂), characteristic of batch culture, the global regulatory system ArcB/ArcA can play an important role in the coordinated control of extracellular superoxide and GSH fluxes and their interaction with intracellular antioxidant systems. The lowest superoxide production was observed in the menA and arcB mutants, while the atpA, atpC and atpE mutants generated superoxide 1.3–1.5 times faster than the parent. The share of exported glutathione in the ubiC, atpA, atpC, and atpE mutants was 2–3 times higher compared to the parent. A high direct correlation (r = 0.87, p = 0.01) between extracellular superoxide and GSH was revealed. The menA and arcB mutants, as well as the cydD mutant lacking the GSH export system CydDC, were not capable of GSH excretion with a decrease in dO₂, which indicates a positive control of GSH export by ArcB. In contrast, ArcB downregulates sodA, therefore, an inverse correlation (r = −0.86, p = 0.013) between superoxide production and sodA expression was observed.

有氧生长的大肠杆菌通过二氢甲萘醌的氧化产生超氧化物通入周质，并同时进行谷胱甘肽（GSH）的连续跨膜循环。在这里，我们表明，在分批培养的特征性溶氧（dO ₂）逐渐减少的条件下，全球调节系统ArcB / ArcA可以在细胞外超氧化物和GSH通量及其调节的协调控制中发挥重要作用。与细胞内抗氧化剂系统的相互作用。在menA和arcB突变体中观察到最低的超氧化物生成量，而atpA，atpC和atpE突变体产生的超氧化物比母体快1.3-1.5倍。出口的谷胱甘肽在ubiC，atpA，atpC和atpE突变体中所占的比例是其亲本的2-3倍。揭示了细胞外超氧化物与GSH之间的高度直接相关性（r = 0.87，p = 0.01）。在中东和北非和arcB突变体，以及所述cydD突变体缺乏GSH出口系统CydDC，没有能力GSH排泄在做减少₂，其指示由ArcB GSH出口的阳性对照。相反，ArcB下调sodA因此，观察到超氧化物产生与sodA表达之间呈负相关（r = -0.86，p = 0.013）。