The antilisterial activity of Thymus vulgaris, Salvia officinalis essential oils (EOs) and bacteriocin bacLP17 (previously isolated from seafood) was determined, using the compounds alone and in combination. The Disk Diffusion, Minimal Inhibitory Concentration (MIC) and Agar Well Diffusion assays were used to evaluate the effectiveness of the compounds against 12 Listeria monocytogenes in planktonic form, whereas the anti-Listeria biofilm activity was determined against the same strains in optical density (O.D.) at 570 nm, with crystal violet staining method. The lowest MIC values resulted for T. vulgaris EO and bacLp17 (0.5 μl/ml and 2 μl/ml, respectively). The combinations with the best results, expressed as FIC-Index, were T. vulgaris/S. officinalis EOs and EOs/bacLp17. The anti-biofilm activity of single EOs and bacLP17 was similar, whereas the combined use of the two kinds of EOs led to a synergistic activity. Lastly, the best anti-biofilm effect was observed with the combination bacLP17/S. officinalis and bacLP17/T. vulgaris, compared to both control and the single use of the EOs. The present study suggests that the combination of natural compounds such as T. vulgaris, S. officinalis EOs and bacLp17 may be a useful approach to the control of planktonic and sessile cells of L. monocytogenes in seafood products.

单独或组合使用化合物，测定了百里香，鼠尾草精油（EOs）和细菌素bacLP17（以前从海鲜中分离）的抗李斯特菌活性。圆盘扩散，最小抑制浓度（MIC）和琼脂井扩散测定法用于评估化合物对浮游形式的12种单核细胞增生李斯特菌的有效性，而抗李斯特菌生物膜活性是针对相同菌株的光密度（OD）确定的）在570 nm下，用结晶紫染色法。寻常锥虫的最低MIC值EO和bacLp17（分别为0.5μl/ ml和2μl/ ml）。与最佳结果的组合，表示为FIC指数，分别为T.寻常/鼠尾草个EO和EOS / bacLp17。单个EO和bacLP17的抗生物膜活性相似，而两种EO的组合使用则产生协同活性。最后，与对照和一次性使用EOs相比，使用bacLP17 / S. officinalis和bacLP17 / T. v。gars的组合观察到最佳的抗生物膜作用。本研究表明，天然化合物如组合寻常T.，鼠尾草个EO和bacLp17可能是的浮游和固着单元的控制的有用方法海鲜产品中的单核细胞增生李斯特氏菌。