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Antibacterial activity and rice-induced resistance, mediated by C15surfactin A, in controlling rice disease caused by Xanthomonas oryzae pv. oryzae
Pesticide Biochemistry and Physiology ( IF 4.2 ) Pub Date : 2020-10-01 , DOI: 10.1016/j.pestbp.2020.104669
Pengfei Jin 1 , Yu Wang 1 , Zheng Tan 1 , Wenbo Liu 1 , Weiguo Miao 1
Affiliation  

Xanthomonas oryzae pv. oryzae (Xoo) is an important pathogen in rice. C15surfactin A, produced by Bacillus velezensis HN-2, displayed antibacterial activity against Xoo and effectively inhibited its infection of rice. The median inhibitory concentration of C15surfactin A was 9.27 μg/mL. Scanning electron and transmission electron microscopy examination showed that C15surfactin A caused significant damage to the cell wall structure of Xoo cells. On the other hand, dramatic increases in the activity of phenylalanine ammonia-lyase (Pal) and H2O2 content were observed in rice leaves inoculated with Xoo from 0 h to 72 h. Quantitative PCR assays indicated that C15surfactin A exposure upregulated the expression of the genes Pr1a, CatA, and Pal. The results showed that C15surfactin A could inhibit the growth of Xoo and effectively induce rice resistance to Xoo by triggering a hypersensitive reaction (HR) via mediation of the activities of antioxidant-related enzymes. Taken together, C15surfactin A has strong antibiotic activity against Xoo and effectively induces rice resistance to Xoo. These results highlight the potential of C15surfactin A as a biocontrol agent against Xoo in rice.

中文翻译:

由 C15surfactin A 介导的抗菌活性和水稻诱导的抗性,在控制由米黄单胞菌引起的水稻病害中。米科

米黄单胞菌 pv。稻瘟病菌(Xoo)是水稻的重要病原菌。由 Bacillus velezensis HN-2 产生的 C15surfactin A 对 Xoo 显示出抗菌活性并有效抑制其对水稻的侵染。C15surfactin A 的中值抑制浓度为 9.27 μg/mL。扫描电镜和透射电镜检查表明,C15surfactin A对Xoo细胞的细胞壁结构造成显着破坏。另一方面,从 0 小时到 72 小时,在接种 Xoo 的水稻叶片中观察到苯丙氨酸解氨酶 (Pal) 的活性和 H2O2 含量急剧增加。定量 PCR 分析表明,C15surfactin A 暴露上调了基因 Pr1a、CatA 和 Pal 的表达。结果表明,C15surfactin A可通过介导抗氧化相关酶的活性触发过敏反应(HR),抑制Xoo的生长,有效诱导水稻对Xoo的抗性。综上所述,C15surfactin A 对 Xoo 具有很强的抗菌活性,可有效诱导水稻对 Xoo 的抗性。这些结果突出了 C15surfactin A 作为水稻 Xoo 生物防治剂的潜力。
更新日期:2020-10-01
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