Tolypocladium guangdongense has a similar metabolite profile to Ophiocordyceps sinensis, a highly regarded fungus used for traditional Chinese medicine with high nutritional and medicinal value. Although the genome sequence of T. guangdongense has been reported, relatively little is known about the regulatory networks for fruiting body development and about the metabolite biosynthesis pathways. In order to address this, an analysis of transcriptome and proteome at differential developmental stages of T. guangdongense was performed. In total, 9076 genes were found to be expressed and 2040 proteins were identified. There were a large number of genes that were significantly differentially expressed between the mycelial stage and the stages. Interestingly, the correlation between the transcriptomic and proteomic data was low, suggesting the importance of the post-transcriptional processes in the growth and development of T. guangdongense. Among the genes/proteins that were both differentially expressed during the developmental process, there were numerous heat shock proteins and transcription factors. In addition, there were numerous proteins involved in terpenoid, ergosterol, adenosine and polysaccharide biosynthesis that also showed significant downregulation in their expression levels during the developmental process. Furthermore, both tryptophan and tryptamine were present at higher levels in the primordium stage. However, indole-3-acetic acid (IAA) levels continuously decreased as development proceeded, and the enzymes involved in IAA biosynthesis were also clearly differentially downregulated. These data could be meaningful in studying the molecular mechanisms of fungal development, and for the industrial and medicinal application of macro-fungi.

广东Totopocladium guangdongense具有与Ophiocordyceps sinensis类似的代谢产物特征，Ophiocordyceps sinensis是一种备受推崇的中药真菌，具有很高的营养和药用价值。尽管已报道了广东冬虫夏草的基因组序列，但对于子实体发育的调控网络和代谢物生物合成途径的了解相对较少。为了解决这个问题，分析了广东红differential不同发育阶段的转录组和蛋白质组。被执行了。总共发现表达了9076个基因，鉴定出2040个蛋白质。在菌丝期与各期之间存在大量显着差异表达的基因。有趣的是，转录组和蛋白质组学数据之间的相关性很低，这表明转录后过程在T. guangdongense生长和发育中的重要性。。在发育过程中均差异表达的基因/蛋白质中，有许多热激蛋白和转录因子。另外，在萜类，麦角固醇，腺苷和多糖生物合成中涉及许多蛋白质，它们在发育过程中也显示出明显的表达下调。此外，色氨酸和色胺在原基期都较高含量存在。但是，吲哚-3-乙酸（IAA）的水平随着发育的进行而持续下降，并且参与IAA生物合成的酶也明显地被差异地下调。这些数据对于研究真菌发育的分子机制以及对大型真菌的工业和医学应用可能是有意义的。