Thermosensitive hydrogels based on polysaccharides are suitable candidates for the design of biodegradable and biocompatible injectable drug delivery systems. Thus, the combination of chitosan (CHI) and β-glycerol phosphate disodium salt (β-GP) has been intensively investigated to develop thermo-induced physical gels. With the aim of exploring the possibilities of optimization of these hydrogels, in this work, chitosan, β-GP and naturally extracted crosslinking agent, genipin (GEN), have been successfully combined, obtaining co-crosslinked hydrogels with both in situ physical and covalent crosslinking. A wide range of β-GP concentrations have been selected in order to analyze its influence on a variety of properties, including gelation time, pore size, water uptake ability, in vitro hydrolytic and enzymatic degradation, mucoadhesion and mechanical and rheological properties. Furthermore, the potential application of the developed systems for the administration and controlled release of an anti-inflammatory anionic drug, such as diclofenac, has been successfully demonstrated.

基于多糖的热敏水凝胶是设计可生物降解和生物相容性可注射药物递送系统的合适候选者。因此，已经深入研究了壳聚糖（CHI）和β-甘油磷酸二钠盐（β-GP）的组合，以开发热诱导的物理凝胶。为了探索优化这些水凝胶的可能性，在这项工作中，壳聚糖，β-GP和天然提取的交联剂genipin（GEN）已成功结合，获得了具有原位物理和共价键的共交联水凝胶交联。选择了广泛的β-GP浓度以分析其对多种特性的影响，包括胶凝时间，孔径，吸水能力，体外水解和酶促降解，粘膜粘附以及机械和流变性能。此外，已经成功地证明了开发的系统用于施用和控制释放消炎性阴离子药物如双氯芬酸的潜在应用。