An efficient in vitro propagation system has been developed for the rapid micropropagation of Thymus serpyllum L. (Banajwain), an aromatic medicinal herb from nodal explant on MS medium. Phenolic leaching and high rate of contamination was the most significant problem in establishing in vitro culture of Thymus serpyllum which was overcome by preparing explants in an antioxidant ascorbic acid (1000 ppm) at 6°C for 45 min and addition of the same antioxidant (50 mgl⁻¹) to the MS medium. The frequency of shoot production was influenced by different cytokinins (Kn, BAP, and Kn + BAP) and 95.56% shoot induction was observed when MS medium was supplemented with 1.0 + 2.0 mgl⁻¹ (Kn + BAP). The maximum average number of shoots 16.93 ± 2.15 and average length (3.98 ± 0.55) was recorded when MS medium have 0.5 + 2.0 mgl⁻¹ (Kn + BAP). The in vitro regenerated microshoots were rooted on MS and half strength MS medium and there was significant difference in root induction on both media under the influence of auxins (IAA, IBA, and NAA). The maximum average number (11.67 ± 3.03) and average root length (3.88 ± 0.71) was reported in half MS medium having 1.0 mgl⁻¹ IBA. The complete regenerated plantlets were acclimatized under growth chamber before transferring to the earthen pots and showed 90% survival.

Graphical abstract

已开发出一种有效的体外繁殖系统，用于快速将雄性百里香百里香（百里香）（一种来自结节外植体的芳香性草药在MS培养基上）快速微繁殖。酚浸出和高污染率是建立百里香百里香的体外培养中最重要的问题，可以通过在抗氧化剂抗坏血酸（1000 ppm）中于6°C制备外植体45分钟并添加相同的抗氧化剂（50）来克服mgl ^-1）至MS培养基。芽的产生频率受不同细胞分裂素（Kn，BAP和Kn + BAP）的影响，当MS培养基中添加1.0 + 2.0 mgl ^-1时，可观察到95.56％的芽诱导。（Kn + BAP）。当MS培养基具有0.5 + 2.0mgl ^-1（Kn + BAP）时，记录了最大平均芽数16.93±2.15和平均长度（3.98±0.55 ）。的体外再生microshoots生根在MS和半强度MS培养基并有在根诱导两种培养基上的植物生长素的影响（IAA，IBA，NAA和）下显著差异。在具有1.0mgl ^- 1IBA的半MS培养基中报道了最大平均数（11.67±3.03）和平均根长（3.88±0.71）。完全再生的幼苗在生长室中适应后再转移到土盆中，并表现出90％的存活率。

图形概要