The five tubulin-binding cofactors (TBC) are involved in tubulin synthesis and the formation of microtubules. Their importance is highlighted by various diseases and syndromes caused by dysfunction or mutation of these proteins. Posttranslational modifications (PTMs) of tubulin promote different characteristics, including stability-creating subpopulations of tubulin. Cell- and time-specific distribution of PTMs has only been investigated in the organ of Corti in gerbils. The aim of the presented study was to investigate the cell type-specific and time-specific expression patterns of TBC proteins and PTMs for the first time in murine cochleae over several developmental stages. For this, murine cochleae were investigated at the postnatal (P) age P1, P7 and P14 by immunofluorescence analysis. The investigations revealed several profound interspecies differences in the distribution of PTMs between gerbil and mouse. Furthermore, this is the first study to describe the spatio-temporal distribution of TBCs in any tissue ever showing a volatile pattern of expression. The expression analysis of TBC proteins and PTMs of tubulin reveals that these proteins play a role in the physiological development of the cochlea and might be essential for hearing.

五种微管蛋白结合辅因子 (TBC) 参与微管蛋白合成和微管形成。由这些蛋白质的功能障碍或突变引起的各种疾病和综合征凸显了它们的重要性。微管蛋白的翻译后修饰 (PTM) 可促进不同的特性，包括稳定的微管蛋白亚群。仅在沙鼠的 Corti 器官中研究了 PTM 的细胞和时间特异性分布。本研究的目的是首次研究小鼠耳蜗在多个发育阶段的 TBC 蛋白和 PTM 的细胞类型特异性和时间特异性表达模式。为此，通过免疫荧光分析对出生后 (P) 年龄 P1、P7 和 P14 的小鼠耳蜗进行了研究。研究揭示了沙鼠和小鼠之间 PTM 分布的几个深刻的种间差异。此外，这是第一项描述 TBC 在任何组织中表现出不稳定表达模式的时空分布的研究。 TBC 蛋白和微管蛋白 PTM 的表达分析表明，这些蛋白在耳蜗的生理发育中发挥作用，并且可能对听力至关重要。