Ethanol has gained its importance as one of the highly demanded biofuels and is being produced from different food crops or food crop wastes after vigorous pretreatment compelling the production process economically incompatible to some extent. The process also becomes non-ecofriendly as the pretreatment process generally produces acid fumes and toxic effluents as well as consumes high energy. In this current study, the potential of the banana peel as a probable substrate for ethanol production was evaluated with the help of logical prediction. Moreover, a single-step ethanol production process was investigated to eliminate hazardous pretreatment processes. A potential bacterial strain SWET4 was isolated from a sewage treatment pond and was identified as a member of the Klebsiella genus by partial sequencing of the 16S rRNA gene. The isolated strain showed ethanol tolerance up to 12% and exhibited its potential as a cellulolytic strain. The bacterial strain SWET4 was found able to ferment banana peel in MSM broth without any other carbon source with a comparatively prolonged lag phase of cellular growth. Trace of ethanol in banana peel fermentation broth of SWET4 was confirmed by high-pressure liquid chromatography. The optimization study for ethanol production enlightened the fact that high temperature and pH supported higher ethanol production. However, due to the growth restriction of the bacterial cell at higher temperatures and pH, only 40 °C and pH 8 were considered optimum. At the optimum substrate concentration (40 g l⁻¹), ethanol concentration was found to be 5.48 g l⁻¹ after 5 days of incubation which summed as a total yield of 0.137 g of ethanol per g of dried banana peel. This current study evaluates the potential of banana peel as a probable substrate for ethanol production and emphasizes on a new possibility of single-step ethanol production without any pretreatment, although metabolic optimization of the strain SWET4 is required to make the process industrially feasible.

乙醇作为一种高度需求的生物燃料已变得越来越重要，在经过严格的预处理后，从不同的粮食作物或粮食作物废料中生产乙醇，在某种程度上使生产过程在经济上不兼容。由于预处理过程通常会产生酸烟和有毒废水，并且消耗大量能量，因此该过程也变得不环保。在当前的研究中，借助逻辑预测评估了香蕉皮作为乙醇生产可能的底物的潜力。此外，研究了一步乙醇生产工艺，以消除有害的预处理工艺。从污水处理池中分离出潜在的细菌菌株SWET4，并将其鉴定为克雷伯菌的成员。属通过对16S rRNA基因进行部分测序来确定。分离出的菌株显示出对乙醇的耐受性高达12％，并显示出其作为纤维素分解菌株的潜力。发现细菌菌株SWET4能够在没有任何其他碳源的情况下在MSM肉汤中发酵香蕉皮，并且细胞生长的滞后阶段相对延长。通过高压液相色谱法确认了SWET4香蕉皮发酵液中的痕量乙醇。乙醇生产的优化研究表明，高温和pH值可以支持更高的乙醇生产。但是，由于细菌细胞在较高温度和pH下的生长受限，因此只有40°C和pH 8被认为是最佳的。在最佳底物浓度（40 g l ^-1）下，发现乙醇浓度为5.48 g l孵育5天后^-1，总和为每克干燥的香蕉皮0.137克乙醇。这项当前的研究评估了香蕉皮作为乙醇生产可能的底物的潜力，并强调了无需任何预处理即可单步生产乙醇的新可能性，尽管需要对菌株SWET4进行代谢优化以使其在工业上可行。