In this study, positively charged polystyrene-co-poly (ar-vinylbenzyl) trimethylammonium nanoparticles loading with Aggregation Induced Emission (AIE) fluorescent molecule sulfonated 9,10-distyrylanthracene derivatives (SDSA) by coulomb force denoted as SDSA/PS-co-PVBTAC have been prepared. The size, size distribution and morphology of nanoparticles have been tested by Dynamic light scattering (DLS) and Scanning electron microscope (SEM). The synthesized nanoparticles possess uniform size, good monodispersity and low biological toxicity. The AIE-type SDSA/PS-co-PVBTAC showed good fluorescence stability at pH from 3 to 10. It has been proved that the biological macromolecules including Bovine Serum Albumin (BSA), DNA and RNA can amplify the cell imaging signals. Moreover, the AIE-type nanoparticles have been shown to perform fluorescent imaging of various cells. It suggests that the prepared SDSA/PS-co-PVBTAC can be used as a new biological probe in the field of cell imaging and detection, as well as expanding the application of AIE fluorescent molecules in biosensing field.

中文翻译：

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带正电荷的聚苯乙烯-共聚（ar-乙烯基苄基）三甲基铵纳米颗粒作为基于聚集诱导发射的各种细胞成像的生物探针

在这项研究中，负载聚集诱导发射 (AIE) 荧光分子的带正电荷的聚苯乙烯-共聚 (ar-乙烯基苄基) 三甲基铵纳米粒子通过库仑力磺化 9,10-二苯乙烯基蒽衍生物 (SDSA)，表示为 SDSA/PS-co-PVBTAC已经准备好了。已通过动态光散射 (DLS) 和扫描电子显微镜 (SEM) 测试了纳米粒子的尺寸、尺寸分布和形态。合成的纳米粒子尺寸均匀、单分散性好、生物毒性低。AIE型SDSA/PS-co-PVBTAC在3至10的pH范围内表现出良好的荧光稳定性。已证明牛血清白蛋白(BSA)、DNA和RNA等生物大分子可以放大细胞成像信号。而且，AIE 型纳米颗粒已被证明可以对各种细胞进行荧光成像。这表明所制备的SDSA/PS-co-PVBTAC可以作为一种新的生物探针应用于细胞成像和检测领域，并拓展了AIE荧光分子在生物传感领域的应用。