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Effect of low-level laser therapy on the inflammatory response in an experimental model of ventilator-induced lung injury.
Photochemical & Photobiological Sciences ( IF 2.7 ) Pub Date : 2020-07-24 , DOI: 10.1039/d0pp00053a Thaís Fernanda Fazza 1 , Bruno Valle Pinheiro 1 , Lídia Maria Carneiro da Fonseca 1 , Luiz Philippe da Silva Sergio 2 , Mateus Pinto Botelho 3 , Gabrielle de Moura Lopes 3 , Flavia de Paoli 4 , Adenilson de Souza da Fonseca 2 , Leda Marília Fonseca Lucinda 5 , Maycon Moura Reboredo 1
Photochemical & Photobiological Sciences ( IF 2.7 ) Pub Date : 2020-07-24 , DOI: 10.1039/d0pp00053a Thaís Fernanda Fazza 1 , Bruno Valle Pinheiro 1 , Lídia Maria Carneiro da Fonseca 1 , Luiz Philippe da Silva Sergio 2 , Mateus Pinto Botelho 3 , Gabrielle de Moura Lopes 3 , Flavia de Paoli 4 , Adenilson de Souza da Fonseca 2 , Leda Marília Fonseca Lucinda 5 , Maycon Moura Reboredo 1
Affiliation
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The effect of low-level laser therapy (LLLT) on an experimental model of ventilator-induced lung injury (VILI) was evaluated in this study. 24 adult Wistar rats were randomized into four groups: protective mechanical ventilation (PMV), PMV + laser, VILI and VILI + laser. The animals of the PMV and VILI groups were ventilated with tidal volumes of 6 and 35 ml kg−1, respectively, for 90 minutes. After the first 60 minutes of ventilation, the animals in the laser groups were irradiated (808 nm, 100 mW power density, 20 J cm−2 energy density, continuous emission mode, and exposure time of 5 s) and after 30 minutes of irradiation, the animals were euthanized. Lung samples were removed for morphological analysis, bronchoalveolar lavage (BAL) and real time quantitative polynucleotide chain reaction (RT-qPCR). The VILI group showed a greater acute lung injury (ALI) score with an increase in neutrophil infiltration, higher neutrophil count in the BAL fluid and greater cytokine mRNA expression compared to the PMV groups (p < 0.05). The VILI + laser group when compared to the VILI group showed a lower ALI score (0.35 ± 0.08 vs. 0.54 ± 0.13, p < 0.05), alveolar neutrophil infiltration (7.00 ± 5.73 vs. 21.50 ± 9.52, p < 0.05), total cell count (1.90 ± 0.71 vs. 4.09 ± 0.96 × 105, p < 0.05) and neutrophil count in the BAL fluid (0.60 ± 0.37 vs. 2.28 ± 0.48 × 105, p < 0.05). Moreover, LLLT induced a decrease in pro-inflammatory and an increase of anti-inflammatory mRNA levels compared to the VILI group (p < 0.05). In conclusion, LLLT was found to reduce the inflammatory response in an experimental model of VILI.
中文翻译：
在呼吸机诱发的肺损伤实验模型中，低水平激光治疗对炎症反应的影响。
在这项研究中评估了低水平激光疗法（LLLT）对呼吸机诱发的肺损伤（VILI）实验模型的影响。将24只成年Wistar大鼠随机分为四组：保护性机械通气（PMV），PMV +激光，VILI和VILI +激光。将PMV和VILI组的动物分别以6和35 ml kg -1的潮气通风90分钟。通气的前60分钟后，照射激光组中的动物（808 nm，功率密度100 mW，20 J cm -2能量密度，连续发射模式和5 s的暴露时间），辐照30分钟后，对动物实施安乐死。取出肺样品进行形态分析，支气管肺泡灌洗（BAL）和实时定量多核苷酸链反应（RT-qPCR）。与PMV组相比，VILI组显示出更高的急性肺损伤（ALI）评分，中性粒细胞浸润增加，BAL液中中性粒细胞计数更高，细胞因子mRNA表达更高（p <0.05）。与VILI组相比，VILI +激光治疗组的ALI评分较低（0.35±0.08对0.54±0.13，p <0.05），肺泡中性粒细胞浸润（7.00±5.73对21.50±9.52，p<0.05），总细胞数（1.90±0.71对比4.09±0.96×10 5，p <0.05）和中性粒细胞计数在BAL液（0.60±0.37对比2.28±0.48×10 5，p <0.05）。此外，与VILI组相比，LLLT诱导了促炎作用的减少和抗炎mRNA的增加（p <0.05）。总之，在VILI实验模型中发现LLLT可以减轻炎症反应。
更新日期：2020-07-24
中文翻译：
在呼吸机诱发的肺损伤实验模型中，低水平激光治疗对炎症反应的影响。
在这项研究中评估了低水平激光疗法（LLLT）对呼吸机诱发的肺损伤（VILI）实验模型的影响。将24只成年Wistar大鼠随机分为四组：保护性机械通气（PMV），PMV +激光，VILI和VILI +激光。将PMV和VILI组的动物分别以6和35 ml kg -1的潮气通风90分钟。通气的前60分钟后，照射激光组中的动物（808 nm，功率密度100 mW，20 J cm -2能量密度，连续发射模式和5 s的暴露时间），辐照30分钟后，对动物实施安乐死。取出肺样品进行形态分析，支气管肺泡灌洗（BAL）和实时定量多核苷酸链反应（RT-qPCR）。与PMV组相比，VILI组显示出更高的急性肺损伤（ALI）评分，中性粒细胞浸润增加，BAL液中中性粒细胞计数更高，细胞因子mRNA表达更高（p <0.05）。与VILI组相比，VILI +激光治疗组的ALI评分较低（0.35±0.08对0.54±0.13，p <0.05），肺泡中性粒细胞浸润（7.00±5.73对21.50±9.52，p<0.05），总细胞数（1.90±0.71对比4.09±0.96×10 5，p <0.05）和中性粒细胞计数在BAL液（0.60±0.37对比2.28±0.48×10 5，p <0.05）。此外，与VILI组相比，LLLT诱导了促炎作用的减少和抗炎mRNA的增加（p <0.05）。总之，在VILI实验模型中发现LLLT可以减轻炎症反应。
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