Skeletal development and its cellular function are regulated by various transcription factors. The T-box (Tbx) family of transcription factors have critical roles in cellular differentiation as well as heart and limbs organogenesis. These factors possess activator and/or repressor domains to modify the expression of target genes. Despite the obvious effects of Tbx20 on heart development, its impact on bone development is still unknown. To investigate the consequence by forced Tbx20 expression in the osteogenic differentiation of human mesenchymal stem cells derived from adipose tissue (Ad-MSCs), these cells were transduced with a bicistronic lentiviral vector encoding Tbx20 and an enhanced green fluorescent protein. Tbx20 gene delivery system suppressed the osteogenic differentiation of Ad-MSCs, as indicated by reduction in alkaline phosphatase activity and Alizarin Red S staining. Consistently, reverse transcription-polymerase chain reaction analyses showed that Tbx20 gain-of-function reduced the expression levels of osteoblast marker genes in osteo-inductive Ad-MSCs cultures. Accordingly, Tbx20 negatively affected osteogenesis through modulating expression of key factors involved in this process. The present study suggests that Tbx20 could inhibit osteogenic differentiation in adipose-derived human mesenchymal stem cells.

中文翻译：

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T - Box20抑制脂肪来源的人间充质干细胞的成骨分化：T - Box20在成骨中的作用

骨骼发育及其细胞功能受多种转录因子调控。T-box（Tbx）家族的转录因子在细胞分化以及心脏和四肢器官发生中起着至关重要的作用。这些因子具有激活子和/或阻遏子结构域，以修饰靶基因的表达。尽管Tbx20对心脏发育有明显的影响，但它对骨骼发育的影响仍然未知。为了研究强制性Tbx20表达在源自脂肪组织（Ad-MSC）的人间充质干细胞成骨分化中的结果，将这些细胞用编码Tbx20的双顺反子慢病毒载体和增强的绿色荧光蛋白进行转导。Tbx20基因递送系统抑制了Ad-MSC的成骨分化，如碱性磷酸酶活性降低和茜素红S染色所示。一致地，逆转录-聚合酶链反应分析表明，Tbx20功能获得降低了骨诱导性Ad-MSCs培养物中成骨细胞标记基因的表达水平。因此，Tbx20通过调节此过程中涉及的关键因子的表达对成骨作用产生负面影响。本研究表明，Tbx20可以抑制脂肪来源的人间充质干细胞的成骨分化。Tbx20通过调节此过程中涉及的关键因子的表达对成骨作用产生负面影响。本研究表明，Tbx20可以抑制脂肪来源的人间充质干细胞的成骨分化。Tbx20通过调节此过程中涉及的关键因子的表达对成骨作用产生负面影响。本研究表明，Tbx20可以抑制脂肪来源的人间充质干细胞的成骨分化。