Glucose oxidase (GOD), an aerobic dehydrogenase, has been used as an antibiotic substitute in feed. A study was conducted to evaluate the differential effects of 2 different GODs fermented by Aspergillus niger or Penicillium amagasakiense on caecal microbiota and to further illuminate the potential roles of changes in the gut microbiota in regulating the growth performance and meat quality of broiler chickens. A total of 420 one-day-old healthy Arbor Acres broilers were randomly assigned to 4 treatments: the control group, the antibiotic growth promoter (AGP) supplementation group, and the GOD-A and GOD-P (GODs produced by A. niger and P. amagasakiense, respectively) groups. As a result, supplementation with GOD produced by P. amagasakiense could significantly improve the average daily weight gain and average daily feed intake of broilers before 21 days of age by significantly increasing the enzymatic activities of jejunal amylase and those of ileal amylase, chymotrypsin, and lipase in 21-day-old broilers and could increase the enzymatic activities of duodenal amylase, jejunal amylase and lipase, and ileal chymotrypsin and lipase in 42-day-old broilers. Meanwhile, compared with AGP treatment, supplementation with GOD produced by P. amagasakiense significantly decreased the L value of 21-day-old broilers and the ΔpH and L* value of 42-day-old broilers, while supplementation with GOD produced by A. niger significantly increased the pH24 h value of 21-day-old and 42-day-old broilers by reducing plasma malondialdehyde content. By using 16S rRNA sequencing, we found that the beneficial bacteria and microbiota in broilers were not disturbed but were improved by GOD supplementation compared with ADP treatment, including the genera Eubacterium and Christensenella and the species uncultured_Eubacterium_sp, Clostridium_asparagiforme, and uncultured_Christensenella_sp, which were positively related to the improved intestinal digestive enzymatic activities, growth performance, and meat quality of broilers. The altered gut microbiota induced by supplementation with glucose oxidase produced by P. amagasakiense mediate better regulatory effects on the meat quality and growth performance of broilers than that induced by supplementation with glucose oxidase produced by A. niger.

中文翻译：

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肠道微生物群的变化介导了黑曲霉和甘崎青霉产生的两种葡萄糖氧化酶对肉鸡肉质和生长性能的不同调节作用。

葡萄糖氧化酶 (GOD) 是一种好氧脱氢酶，已被用作饲料中的抗生素替代品。进行了一项研究，以评估由黑曲霉或青霉 amagasakiense 发酵的 2 种不同 GOD 对盲肠微生物群的不同影响，并进一步阐明肠道微生物群变化在调节肉鸡生长性能和肉质方面的潜在作用。总共 420 只 1 日龄健康的 Arbor Acres 肉鸡被随机分配到 4 个处理组：对照组、抗生素生长促进剂 (AGP) 补充组以及 GOD-A 和 GOD-P（由 A. niger 生产的 GODs）和 P. amagasakiense，分别）组。结果，补充了 P 产生的 GOD。amagasakiense可显着提高21日龄肉鸡空肠淀粉酶和回肠淀粉酶、糜蛋白酶和脂肪酶的酶活性，显着提高21日龄前肉鸡的平均日增重和平均日采食量，并可增加42 日龄肉鸡十二指肠淀粉酶、空肠淀粉酶和脂肪酶以及回肠胰凝乳蛋白酶和脂肪酶的酶活性。同时，与AGP处理相比，补充甘崎假单胞菌产生的GOD显着降低了21日龄肉鸡的L值和42日龄肉鸡的ΔpH和L*值，而补充A. amagasakiense产生的GOD。尼日尔通过降低血浆丙二醛含量显着提高了21日龄和42日龄肉鸡的pH24 h值。通过使用16S rRNA测序，我们发现，与 ADP 处理相比，添加 GOD 后肉鸡的有益细菌和微生物群没有受到干扰，反而有所改善，包括真杆菌属和克里斯滕森氏菌属以及未培养的_真杆菌_sp、梭状芽胞杆菌_天门冬杆菌和未培养的_克里斯滕森氏菌_sp，它们与肠道消化酶的改善呈正相关肉鸡的活动、生长性能和肉质。与补充黑曲霉产生的葡萄糖氧化酶相比，通过补充 P. amagasakiense 产生的葡萄糖氧化酶诱导的肠道微生物群改变对肉鸡的肉质和生长性能具有更好的调节作用。包括Eubacterium属和Christensenella属以及uncultured_Eubacterium_sp、Clostridium_asparagiforme和uncultured_Christensenella_sp种，它们与提高肉鸡肠道消化酶活性、生长性能和肉质呈正相关。与补充黑曲霉产生的葡萄糖氧化酶相比，通过补充 P. amagasakiense 产生的葡萄糖氧化酶诱导的肠道微生物群改变对肉鸡的肉质和生长性能具有更好的调节作用。包括Eubacterium属和Christensenella属以及uncultured_Eubacterium_sp、Clostridium_asparagiforme和uncultured_Christensenella_sp种，它们与提高肉鸡肠道消化酶活性、生长性能和肉质呈正相关。与补充黑曲霉产生的葡萄糖氧化酶相比，通过补充 P. amagasakiense 产生的葡萄糖氧化酶诱导的肠道微生物群改变对肉鸡的肉质和生长性能具有更好的调节作用。和肉鸡的肉质。与补充黑曲霉产生的葡萄糖氧化酶相比，通过补充 P. amagasakiense 产生的葡萄糖氧化酶诱导的肠道微生物群改变对肉鸡的肉质和生长性能具有更好的调节作用。和肉鸡的肉质。与补充黑曲霉产生的葡萄糖氧化酶相比，通过补充 P. amagasakiense 产生的葡萄糖氧化酶诱导的肠道微生物群改变对肉鸡的肉质和生长性能具有更好的调节作用。