A light pink coloured bacterial strain ERGS5:01 isolated from glacial stream water of Sikkim Himalaya was affiliated to Janthinobacterium lividum based on 16S rRNA gene sequence identity and phylogenetic clustering. Whole genome sequencing was performed for the strain to confirm its taxonomy as it lacked the typical violet pigmentation of the genus and also to decipher its survival strategy at the aquatic ecosystem of high elevation. The PacBio RSII sequencing generated genome of 5,168,928 bp with 4575 protein-coding genes and 118 RNA genes. Whole genome-based multilocus sequence analysis clustering, in silico DDH similarity value of 95.1% and, the ANI value of 99.25% established the identity of the strain ERGS5:01 (MCC 2953) as a non-violacein producing J. lividum. The genome comparisons across genus Janthinobacterium revealed an open pan-genome with the scope of the addition of new orthologous cluster to complete the genomic inventory. The genomic insight provided the genetic basis of freezing and frequent freeze-thaw cycle tolerance and, for industrially important enzymes. Extended insight into the genome provided clues of crucial genes associated with adaptation in the harsh aquatic ecosystem of high altitude.

中文翻译：

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从产生非堇青素的淡紫色Janthinobacterium lividum ERGS5：01的高质量草图基因组揭示了高海拔适应策略

基于16S rRNA基因序列同一性和系统发生聚类，从锡金喜马拉雅山冰川水分离出的浅粉色细菌菌株ERGS5：01隶属于淡淡詹氏杆菌。对菌株进行了全基因组测序，以确认其分类，因为该菌株缺乏典型的紫色色素沉着，并破译了其在高海拔水生生态系统中的生存策略。PacBio RSII测序产生了5168928 bp的基因组，其中包含4575个蛋白质编码基因和118个RNA基因。基于全基因组的多基因座序列分析聚类，在计算机上DDH相似度值为95.1％，ANI值为99.25％，确定了菌株ERGS5：01（MCC 2953）为非产紫堇青素的青枯菌。詹氏菌属之间的基因组比较揭示了一个开放的全基因组，其范围包括添加新的直系同源簇以完成基因组清单。基因组学的见解为冷冻和频繁的冻融循环耐受性以及工业上重要的酶提供了遗传基础。对基因组的深入了解提供了与高海拔恶劣水生生态系统适应相关的关键基因的线索。