SNARE (soluble N-ethyl maleimide sensitive factor attachment protein receptor) mediates membrane fusion of vesicle transport in eukaryotic cells. LjSYP132s is a member of Qa-SNAREs in Lotus japonicus, and two isoforms, LjSYP132a and LjSYP132b, are generated by alternative splicing. Immunoblot analysis showed LjSYP132s antibody was expressed strongly in infected root nodules and seeds by post-transcriptional modification. In LjSYP132a or LjSYP132b silencing roots (RNAi-LjSYP132a, RNAi-LjSYP132b), infection thread (IT) was not elongated, suggesting that both LjSYP132a and LjSYP132b have a role in IT progression. The results were consistent with the data of qRT-PCR that both genes expressed at the early stage of infection. However, during the nodulation only LjSYP132a was induced. LjSYP132s protein was observed in the M. loti-inoculated roots of mutants, nfr1, castor, and pollux, suggesting that LjSYP132s can be induced without Nod factor signaling. Accumulation of LjSYP132s in the PBM suggests the function of not only IT formation but also nutrient transport. In contrast, qRT-PCR showed that LjSYP132b was expressed in the seeds. A stable transgenic plant of LjSYP132b, R132b, was produced by RNAi silencing. In the R132b plants, small pods with a few seeds and abnormal tip growth of the pollen tubes were observed, suggesting LjSYP132b has a role in pollen tube growth as well as nutrient transport in the plasma membrane of seeds.

中文翻译：

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Qa-SNARE LjSYP132s对日本莲的结瘤和种子发育的要求。

SNARE（可溶性N-乙基马来酰亚胺敏感因子附着蛋白受体）介导真核细胞中囊泡转运的膜融合。LjSYP132s是Lotus japonicus中Qa-SNARE的成员，并且通过替代剪接生成了两个同工型LjSYP132a和LjSYP132b。免疫印迹分析表明，通过转录后修饰，LjSYP132s抗体在感染的根瘤和种子中强烈表达。在LjSYP132a或LjSYP132b沉默根（RNAi-LjSYP132a，RNAi-LjSYP132b）中，感染线（IT）未延长，表明LjSYP132a和LjSYP132b在IT进步中发挥作用。结果与qRT-PCR的数据一致，这两个基因均在感染初期表达。然而，在结瘤期间仅诱导了LjSYP132a。LjSYP132s蛋白在突变体nfr1，castor和pollux接种的M. loti接种根中观察到，表明LjSYP132s可以在没有Nod因子信号转导的情况下被诱导。LjSYP132s在PBM中的积累表明，它不仅具有IT形成的功能，而且具有养分转运的功能。相反，qRT-PCR显示LjSYP132b在种子中表达。LjSYP132b的稳定转基因植物R132b是通过RNAi沉默产生的。在R132b植物中，观察到带有少量种子的小荚果，并且花粉管的尖端生长异常，这表明LjSYP132b在花粉管生长以及种子质膜中的养分转运中具有作用。