ABSTRACT The effect of Citrus stubborn disease (CSD), caused by Spiroplasma citri, on chemical composition and antimicrobial activities of the essential oils of (Citrus sinensis (L) Osbeck) was studied. The results showed that the essential oil extracted from both healthy and Citrus fruit infected by the stubborn disease did not have the same physicochemical characteristics, but have identical chemical composition, with octanal, β-phellandrene, d-limonene, 1 r-α pinene, and d-limonene as major compounds. The infected fruits contained new toxic compounds (2-cyclohexen-1-ol, 2-methyl-5-(1-methylethenyl)-cis, decanal, trans-p-mentha-2, 8-dienol, 2-cyclohexen-1-ol, and, 1-methyl-4-[1-methy-4-(1-methyletheyl)]-cis), at low concentrations (<1%). Both essential oils showed very interesting antibacterial activity against Escherichia coli ATCC 25922, Staphylococcus aureus ATCC 25923, and Pseudomonas aeruginosa ATCC 27853. However, they did not exert any antifungal activity against Botrytis cinerea. The minimum inhibitory concentration (MIC) for the bacterial strains, which were sensitive to the essential oil extracted from both infected and uninfected C. sinensis, was of 4 µL and 32 µL/mL, respectively.

中文翻译：

---

柑橘顽固病（Spiroplasma Citri）对橙（Citrus Sinensis (L) Osbeck）精油果实化学成分的影响

摘要 研究了由柑橘螺旋体引起的柑橘顽固病 (CSD) 对 (Citrus sinensis (L) Osbeck) 精油的化学成分和抗菌活性的影响。结果表明，从受顽固病害感染的健康和柑橘类水果中提取的精油没有相同的理化特性，但具有相同的化学成分，具有辛醛、β-水芹烯、d-苎烯、1r-α蒎烯、和 d-柠檬烯作为主要化合物。受感染的果实含有新的有毒化合物（2-cyclohexen-1-ol, 2-methyl-5-(1-methylethenyl)-cis, decanal, trans-p-mentha-2, 8-dienol, 2-cyclohexen-1- ol 和 1-甲基-4-[1-甲基-4-(1-甲基乙基)]-顺式)，低浓度 (<1%)。两种精油都对大肠杆菌 ATCC 25922 显示出非常有趣的抗菌活性，金黄色葡萄球菌 ATCC 25923 和铜绿假单胞菌 ATCC 27853。但是，它们对灰葡萄孢菌没有任何抗真菌活性。细菌菌株的最低抑菌浓度 (MIC) 分别为 4 µL 和 32 µL/mL，对从感染和未感染的中华白菜中提取的精油敏感。