ABSTRACT This study aimed to evaluate the effects of cryoprotectant agents on Passiflora eichleriana, Passiflora crystallina and Passiflora nitida seeds undergoing cryopreservation. The experiment was conducted with non-cryoprotected or non-cryopreserved seeds (control), cryopreserved seeds without cryoprotectant and cryopreserved seeds treated with the following cryoprotectants: 1.73, 2.28, 2.60 or 2.71 mol glycerol; 0.37, 0.46, 0.54 or 0.61 mol sucrose; or 0.37, 0.72, 1.04 or 1.35 mol dimethylsulfoxide (DMSO). After treatment, the seeds were cryopreserved in liquid nitrogen (LN2) for 7 days and then defrosted in 37°C water for 5 min. The seeds were submitted to germination and vigor tests consisting of four replicates of 50 seeds. Percentage of germination (PE) and germination velocity index, percentage of emergence velocity index, shoot and root length, and shoot and root dry mass were evaluated. After cryopreservation, all of germination and vigor values were higher without the use of cryoprotectants. The cryoprotectant glycerol had a deleterious effect on seed viability and vigor for all Passiflora species. Glycerol inhibit seedling emergence and concentrations higher than 2.60 mol seed germination on P. cristalina. Higher concentrations of DMSO decrease seed viability and vigor in P. crystalina and P. nitida.

中文翻译：

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使用冷冻保护剂对百香果进行冷冻保存的功效

摘要 本研究旨在评估冷冻保护剂对西番莲、结晶西番莲和西番莲种子进行冷冻保存的影响。使用未冷冻保护或未冷冻保存的种子（对照）、没有冷冻保护剂的冷冻保存种子和用以下冷冻保护剂处理的冷冻保存种子进行实验：1.73、2.28、2.60 或 2.71 mol 甘油；0.37、0.46、0.54或0.61摩尔蔗糖；或 0.37、0.72、1.04 或 1.35 mol 二甲亚砜 (DMSO)。处理后，种子在液氮 (LN2) 中冷冻保存 7 天，然后在 37°C 水中解冻 5 分钟。对种子进行发芽和活力测试，由 50 粒种子的四次重复组成。发芽率（PE）和发芽速度指数，出苗速度指数的百分比，评价地上部和根的长度，以及地上部和根的干重。冷冻保存后，在不使用冷冻保护剂的情况下，所有的发芽值和活力值都较高。冷冻保护剂甘油对所有西番莲物种的种子活力和活力具有有害影响。甘油抑制幼苗出苗，浓度高于 2.60 mol 的 P. cristalina 种子萌发。较高浓度的 DMSO 会降低 P. crystalina 和 P. nitida 的种子活力和活力。克里斯塔利娜。较高浓度的 DMSO 会降低 P. crystalina 和 P. nitida 的种子活力和活力。克里斯塔利娜。较高浓度的 DMSO 会降低 P. crystalina 和 P. nitida 的种子活力和活力。