ABSTRACT

The effects of straw input, including three strategies: chopped straw, straw-derived compost and biochar, on bacterial communities and chemical composition in maize rhizosphere were investigated according to a three-year field experiment. Illumina MiSeq sequencing showed that biochar input increased bacterial richness but decreased bacterial diversity in rhizosphere when compared with the results from no straw control. The functional prediction of Kyoto Encyclopedia of Genes and Genome pathways confirmed that soil subjected to straw input changed the abundance of microbiomes involved in carbohydrate metabolism significantly. Metabolomics analysis showed that straw input changed the metabolite profile of the rhizosphere soil of maize, which was most evident in the treatment of chopped-straw input. The match between the global metabolic profiles and bacterial distribution patterns was weak by performing Procrustes analysis (m² = 0.851, R = 0.386, P < 0.05). However, the associations between the special members of genera, predicted function involved in carbohydrate metabolism, and rhizosphere metabolites, including sugars and organic acid, were significant (P < 0.05).

摘要

根据一项为期三年的田间试验，研究了秸秆输入对包括玉米切碎，秸秆衍生的堆肥和生物炭三种策略对玉米根际细菌群落和化学成分的影响。Illumina MiSeq测序显示，与无秸秆控制的结果相比，生物炭输入增加了根际细菌的丰富度，但减少了细菌多样性。京都基因百科全书和基因组途径的功能预测证实，秸秆输入的土壤显着改变了参与碳水化合物代谢的微生物群的丰度。代谢组学分析表明，秸秆输入改变了玉米根际土壤的代谢产物谱，这在切碎秸秆输入的处理中最为明显。² = 0.851，R = 0.386，P <0.05）。然而，属的特殊成员，参与碳水化合物代谢的预测功能与包括糖和有机酸在内的根际代谢产物之间的关联很显着（P <0.05）。