Abstract Andrographolide sodium bisulfite (ASB) widely used for intestinal infections and respiratory tract infections. It has been reported to frequently cause acute renal failure in clinical practice. The purpose of this study was to evaluate the nephrotoxicity and toxicokinetics in Sprague-Dawley rats after a single-dose injection of ASB administered at 100, 600, and 1000 mg/kg via intravenous tail injections. The concentrations in plasma and kidney microdialysates were analyzed by high-performance liquid chromatography. Blood urea nitrogen and creatinine levels in plasma were determined and toxicokinetic parameters were observed. In plasma and kidney, the elimination constant and clearance were decreased and the half-time was increased with increasing dose. Blood urea nitrogen and creatinine were positively correlated with ASB concentration indicating the potential for accumulation in kidney that can eventually lead to damage.

中文翻译：

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大鼠肾微透析液中穿心莲内酯亚硫酸氢钠的测定及毒代动力学研究

摘要 穿心莲内酯亚硫酸氢钠（ASB）广泛用于肠道感染和呼吸道感染。据报道，它在临床实践中经常引起急性肾功能衰竭。本研究的目的是评估 Sprague-Dawley 大鼠通过静脉尾部注射单剂量注射 100、600 和 1000 mg/kg 的 ASB 后的肾毒性和毒代动力学。通过高效液相色谱分析血浆和肾脏微透析液中的浓度。测定血浆中的血尿素氮和肌酐水平并观察毒代动力学参数。在血浆和肾脏中，随着剂量的增加，消除常数和清除率降低，半衰期增加。