Abstract The main aim of this study was to histochemically estimate the relative copper content in the liver during the breeding cycle of female Ohrid trout, a wild population endemic to the Ohrid Lake. The histology of the oogenetic cycle in this species was documented for the first time. Histochemistry with semi-quantification revealed positive correlations between the gonadosomatic indices and copper load. Uptake of the metal increases in the liver as the ovaries increase in size, with the highest hepatic copper load occurring postspawning. The plasmatic levels of 17β-oestradiol were also quantified, but no correlations to copper load were found, so there is no direct estrogenic control on the uptake and release of copper from the bloodstream to either organ. Therefore, when interpreting field studies or assays on hepatic copper pollution and toxicity, breeding-related seasonal changes in Ohrid trout must be taken into account.

中文翻译：

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与卵巢成熟周期相关的 Ohrid 鳟鱼肝细胞铜负荷的变化

摘要 本研究的主要目的是通过组织化学方法估计雌性奥赫里德鳟鱼（一种奥赫里德湖特有的野生种群）繁殖周期中肝脏中的相对铜含量。首次记录了该物种卵子发育周期的组织学。半定量组织化学显示性腺指标与铜负荷之间呈正相关。随着卵巢增大，肝脏对金属的吸收增加，产卵后肝脏铜含量最高。17β-雌二醇的血浆水平也被量化，但没有发现与铜负荷的相关性，因此没有直接的雌激素控制铜从血流到任一器官的吸收和释放。所以，