Abstract Wooden sleepers used in railways are impregnated with preservatives with fungicidic and bactericidic properties, which contain a large amount of harmful polycyclic aromatic hydrocarbons (PAHs). The aim of this research was to reveal the type of fungi in the rhizosphere of plants, which tolerate pollutants and can be involved in bioremediation process of creosote-contaminated used wooden railway sleepers. Tagetes patula grown in the substrate with 12.5% creosote impregnated used wooden sleepers chips additive is characterized by the most intensive root development and the biggest population of active fungi, therefore it is recommended for further investigation. Number of colonies of fungi in the rhizosphere of investigated plants decreased in many cases by increasing the amount of sleepers chips additive in substrate, however it was greater in the substrates with creosote treated wooden sleepers chips additives (12.5, 25 and 37.5% wooden sleepers chips) than in the pure substrate and various substrates with additives of pure Pinus sylvestris chips. This is a pioneering study searching for the local fungi species in Lithuania, which can be further utilized for the bioremediation of PAHs.

中文翻译：

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杂酚油污染底物对植物根际生物修复潜力微观真菌的影响

摘要 铁路用木枕浸渍了具有杀菌、杀菌作用的防腐剂，其中含有大量有害的多环芳烃（PAHs）。本研究的目的是揭示植物根际中的真菌类型，它们耐受污染物并可以参与被杂酚油污染的旧木轨枕的生物修复过程。在含有 12.5% 杂酚油浸渍的旧木枕木屑添加剂的基质中生长的万寿菊具有最密集的根系发育和最大的活性真菌种群，因此建议进一步研究。在许多情况下，通过增加基质中枕木屑添加剂的量，被调查植物根际真菌的菌落数量减少，然而，在含有杂酚油处理过的木枕木屑添加剂的基材中（12.5%、25% 和 37.5% 木枕木屑），其含量高于纯基材和各种含有纯樟子松木屑添加剂的基材。这是在立陶宛寻找当地真菌物种的开创性研究，可进一步用于多环芳烃的生物修复。