ABSTRACT Constructing satisfied small-diameter vascular graft (diameter less than 6 mm) remains an unsolvable challenge in vascular tissue engineering. This study described the fabrication of electrospun polyurethane/polycaprolactone (PU/PCL) membranes chemically grafted with various densities of conjugated linoleic acid (CLA) – an antithrombotic fatty acid – for making small-diameter blood vessel. Differences in mechanical, antithrombotic properties and biocompatibility of the membranes resulting from the CLA-grafting procedure were the focus of the study. Investigation of mechanical properties relevant to vascular graft application revealed that these properties of the membranes remained unaffected and satisfied clinical criteria following the CLA graft. Blood–membrane interaction assays showed that the CLA-grafted membranes mitigated the adhesion of blood cells, as well as preventing blood coagulation. These effects were also commensurate with increasing density of CLA, suggesting an effective approach to improve antithromboticity. Cellular tests suggested that CLA has an optimal density at which it promoted cell proliferation on the surface of the membranes; however, excessive presence of CLA might cause undesirable inhibition on cells. In conclusion, PU/PCL membrane grafted with CLA could be a prospective material for vascular tissue engineering with further development and investigation.

中文翻译：

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用于人工血管移植应用的共轭亚油酸接枝聚氨酯/聚己内酯膜

摘要 构建令人满意的小直径血管移植物（直径小于 6 mm）仍然是血管组织工程中无法解决的挑战。这项研究描述了电纺聚氨酯/聚己内酯 (PU/PCL) 膜的制造，该膜化学接枝了各种密度的共轭亚油酸 (CLA)——一种抗血栓形成的脂肪酸——用于制造小直径血管。由 CLA 移植程序导致的膜在机械、抗血栓形成和生物相容性方面的差异是研究的重点。对与血管移植物应用相关的机械性能的研究表明，在 CLA 移植后，这些膜的性能不受影响并满足临床标准。血膜相互作用分析表明，CLA 移植膜减轻了血细胞的粘附，并防止了血液凝固。这些作用也与 CLA 密度的增加相称，表明是一种改善抗血栓形成的有效方法。细胞试验表明，CLA 具有促进细胞膜表面细胞增殖的最佳密度；然而，CLA 的过量存在可能会导致对细胞的不良抑制。总之，接枝CLA的PU/PCL膜有望成为血管组织工程的一种有前景的材料，有待进一步的发展和研究。细胞试验表明，CLA 具有促进细胞膜表面细胞增殖的最佳密度；然而，CLA 的过量存在可能会导致对细胞的不良抑制。总之，接枝CLA的PU/PCL膜有望成为血管组织工程的一种有前景的材料，有待进一步的发展和研究。细胞试验表明，CLA 具有促进细胞膜表面细胞增殖的最佳密度；然而，CLA 的过量存在可能会导致对细胞的不良抑制。总之，接枝CLA的PU/PCL膜有可能成为血管组织工程的一种有前景的材料，有待进一步发展和研究。