ABSTRACT This study proposes to develop a dual-acting antibacterial film of porous chitosan (Cs) embedded with small molecular compound, which possesses photosensitive characteristics with bactericidal efficacy, to promote the accelerated recovery of infectious wounds. The Cs/small molecular compound (Cs-cpd.2) dressing was prepared using the freeze-drying method. Characterization of the synthesized Cs-cpd.2 indicated that it has high porosity and moisture absorption effect, hence enhancing the absorption of wound exudate. Experimental results showed that Cs-cpd.2 dressing has good bactericidal and bacteriostatic effects on Staphylococcus aureus under visible-light irradiation and has antibacterial effect in the dark. It was also found that the small molecular compound does not have cytotoxicity at a dose of 0–5 μM. Furthermore, Cs-cpd.2 that contained small molecular compound with a concentration of 0.3–1 μM has positive effect on both the cell viability rate and cell proliferation rate of human fibroblast CG1639. Cs-cpd.2 can significantly promote cell proliferation when the small molecular compound and the basic fibroblast growth factor bFGF were added together. Therefore, the proposed Cs-cpd.2 dressing is feasible for photodynamic therapy (PDT) and clinical wound dressing applications.

中文翻译：

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嵌入光敏剂的双效抗菌多孔壳聚糖膜

摘要本研究提出开发一种嵌入小分子化合物的多孔壳聚糖（Cs）双作用抗菌膜，具有光敏特性和杀菌功效，以促进感染性伤口的加速恢复。Cs/小分子化合物（Cs-cpd.2）敷料采用冷冻干燥法制备。合成的Cs-cpd.2的表征表明它具有高孔隙率和吸湿效果，从而增强了伤口渗出液的吸收。实验结果表明，Cs-cpd.2敷料在可见光照射下对金黄色葡萄球菌具有良好的杀菌抑菌作用，在黑暗中具有抗菌作用。还发现该小分子化合物在 0-5 μM 的剂量下没有细胞毒性。此外，Cs-cpd。含有浓度为 0.3-1 μM 的小分子化合物的 2 对人成纤维细胞 CG1639 的细胞存活率和细胞增殖率均具有积极影响。当小分子化合物和碱性成纤维细胞生长因子bFGF一起加入时，Cs-cpd.2能显着促进细胞增殖。因此，所提出的 Cs-cpd.2 敷料对于光动力疗法 (PDT) 和临床伤口敷料应用是可行的。