ABSTRACT 2D sections of rice (Oryza sativa L.) mesophyll cells are complex and the cell interior contains a large volume of chloroplasts. Furthermore, the structure of chloroplasts changes in response to salt stress. In this study, we used a 3D reconstruction method based on serial section light microscopy to analyze a wide range of structures in leaf tissues and compared the intracellular structure of mesophyll cells of rice under normal and salt-treated conditions. The 3D reconstructed models revealed that mesophyll cells appeared as ellipsoid discs with several lobes around the cell periphery, with the volumes showing no significant difference between control and salt-treated plants. The chloroplast structure in the whole mesophyll cell was altered under salt stress, showing a reduced coverage area, whereas their volumes did not differ between treatments. These findings suggest that 3D reconstruction based on serial light micrographs can reveal the morphology of cells and chloroplasts in plant tissue. Graphical abstract

中文翻译：

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连续切片光学显微镜对水稻叶片组织中叶肉细胞的三维解剖

摘要 水稻（Oryza sativa L.）叶肉细胞的二维切片很复杂，细胞内部含有大量叶绿体。此外，叶绿体的结构会因盐胁迫而发生变化。在这项研究中，我们使用基于连续切片光学显微镜的 3D 重建方法来分析叶组织中的各种结构，并比较正常和盐处理条件下水稻叶肉细胞的细胞内结构。3D 重建模型显示，叶肉细胞呈椭圆盘状，细胞外围有几个裂片，对照植物和盐处理植物之间的体积没有显着差异。整个叶肉细胞中的叶肉结构在盐胁迫下发生了改变，显示出覆盖面积减少，而它们的体积在处理之间没有差异。这些发现表明，基于连续光显微照片的 3D 重建可以揭示植物组织中细胞和叶绿体的形态。图形概要