ABSTRACT Prohydrojasmon (PDJ) is a synthetic derivative of the plant hormone jasmonic acid (JA), which is used in apples, grapes, and mandarin oranges as a plant growth regulator. Here, we show that irrigation of PDJ could prevent spindly growth of rice seedlings in both indica and japonica cultivars. PDJ also inhibited root elongation in hydroponically growing seedlings in various cultivars. The higher dry root weight following PDJ application than the controls was observed in two japonica cultivars. Furthermore, its reduction was slight in comparison with the inhibition of root elongation. Starch degradation in the endosperm of seedlings grown in PDJ solution was slower than those grown in the control. The inhibition of root elongation could be mediated by the combined application of PDJ and appropriate concentrations of a synthetic auxin, NAA (1-Naphthaleneacetic acid). Additionally, we generated transgenic rice carrying sGFP driven by the PBZ1 promoter, a marker gene for abiotic and biotic stress responses. The expression of PBZ1p::sGFP was induced by PDJ treatment in the roots and seeds. PDJ could be used as a plant growth regulator to prevent spindly growth and to induce stress responses in rice seedlings.

中文翻译：

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Prohydrojasmon 阻止细长生长并诱导非生物和生物胁迫标记基因 PBZ1p::sGFP 在水稻中的表达

摘要 Prohydrojasmon (PDJ) 是植物激素茉莉酸 (JA) 的合成衍生物，用于苹果、葡萄和橘子作为植物生长调节剂。在这里，我们表明 PDJ 的灌溉可以防止籼稻和粳稻品种中水稻幼苗的细长生长。PDJ 还抑制了各种栽培品种中水培幼苗的根伸长。在两个粳稻品种中观察到施用 PDJ 后的干根重量高于对照。此外，与根伸长的抑制相比，它的减少是轻微的。在 PDJ 溶液中生长的幼苗胚乳中的淀粉降解比在对照中生长的慢。根伸长的抑制可以通过 PDJ 和适当浓度的合成生长素的联合应用介导，NAA（1-萘乙酸）。此外，我们生成了携带由 PBZ1 启动子驱动的 sGFP 的转基因水稻，PBZ1 是一种非生物和生物胁迫反应的标记基因。PBZ1p::sGFP 在根和种子中通过 PDJ 处理诱导表达。PDJ 可用作植物生长调节剂，以防止细长生长并诱导水稻幼苗的逆境反应。