ABSTRACT

Red Banded Mango Caterpillar (RBMC), Deanolis sublimbalis Snellen (Lepidoptera: Crambidae) is a serious pest of mango fruits in several regions of South and South East Asia. Molecular characterisation of twenty nine different populations was done using the mitochondrial gene, Mitochondrial Cytochrome Oxidase-I (mtCOI) and nuclear gene, Elongation Factor 1 Alpha (EF-1α) to estimate the genetic variability among the RBMC populations. Nucleotide analysis of genetic diversity and phylogenetic analysis of the mtCOI, EF-1α and concatenated sequences (both COI and EF-1α) indicated that the RBMC populations from different geographical regions used in this study are homogenous. The nucleotide diversity (π) and overall mean distance for mtCOI and EF-1α were 0.003635, 0.004 ± 0.001, and 0.00, 0.00, respectively. Moreover, nucleotide diversity and overall mean distance of concatenated sequences were π = 0.009809 and 0.010 ± 0.002. Four mtCOI gene sequences of RBMC (Two from India, Two from Canada) from GenBank were juxtaposed with Indian test populations while building phylogenetic tree indicated that the RBMC from India and Canada are less divergent. With respect to mtCOI, no significant molecular diversity within the RBMC populations of different geographical locations of India as well as Canada was found. However, the phylogenetic tree implied the probability of emerging genetic differences in RBMC populations.

摘要

红带芒果毛毛虫（RBMC），Deanolis sublimbalis Snellen（鳞翅目：Crombidae）是芒果果实的一种严重害虫，在东南亚和东南亚的一些地区。使用线粒体基因，线粒体细胞色素氧化酶-I（mtCOI）和核基因，延伸因子1α（EF-1α）对29个不同种群进行了分子表征，以评估RBMC种群之间的遗传变异性。mtCOI，EF-1α和串联序列（COI和EF-1α两者）的遗传多样性核苷酸分析和系统发育分析）指出，本研究使用的来自不同地理区域的RBMC种群是同质的。mtCOI和EF-1α的核苷酸多样性（π）和总体平均距离分别为0.003635、0.004 ±0.001和0.00、0.00。此外，连接序列的核苷酸多样性和总平均距离为π= 0.009809和0.010±0.002。来自GenBank的RBMC的四个mtCOI基因序列（两个来自印度，两个来自加拿大）与印度测试种群并列，同时建立的系统树表明，印度和加拿大的RBMC的差异较小。关于mtCOI，在印度和加拿大不同地理位置的RBMC种群中未发现明显的分子多样性。但是，系统发育树暗示了RBMC群体中出现遗传差异的可能性。