Abstract

Three yeast strains (Hue-1, Hue-8, and Hue-19) with strong heavy metal tolerance were isolated from mangosteen from Hue city, Vietnam. They exhibited identical phenotype and phylogeny. Sequence analysis of the D1/D2 region of the LSU rRNA gene and the internal transcribed spacer (ITS) region demonstrated that the closest relative of these strains is Papiliotrema sp. with 2.12% and 3.55–3.7% divergence in the D1/D2 domain, and ITS domain, respectively. Based on the physiological, biochemical, and molecular data, the three strains belong to a novel species of Papiliotrema genus, for which the name Papiliotrema huenov sp. nov. is proposed. These strains are highly tolerant of heavy metals compared to other yeasts, being able to grow in the presence of 2 mM Pb (II), 2 mM Cd (II), and up to 5 mM Ni (II), but no growth was observed in the presence of 1 mM As (III).

摘要

从越南顺化市的山竹中分离出三种具有较强重金属耐受性的酵母菌株（Hue-1，Hue-8和Hue-19）。他们表现出相同的表型和系统发育。LSU rRNA基因的D1 / D2区和内部转录间隔区（ITS）区的序列分析表明，这些菌株的近亲是Papiliotrema sp。D1 / D2域和ITS域的差异分别为2.12％和3.55-3.7％。基于生理，生化和分子数据，该三种菌株属于一个新种Papiliotrema属，为其名称Papiliotrema huenovsp。十一月 被提议。与其他酵母菌相比，这些菌株对重金属的耐受性高，能够在2 mM Pb（II），2 mM Cd（II）和最高5 mM Ni（II）的存在下生长，但未观察到生长在1 mM As（III）存在下。