ABSTRACT

Salvadora persica L. is a medicinal plant distributed in desert and subtropical regions. In Rabigh, the few persisting populations of this plant are exposed to overcutting, overgrazing and, recently, human habitation. The conservation of S. persica requires an assessment of their genetic diversity and genetic differentiation. We applied 40 simple sequence repeat (SSR) primers, with which 22 polymorphic loci were identified. The PIC values were varied between 0.858 and 0.967. 240 plant accessions were sampled from four sites in Rabigh: Wadi EL Khaneg, Wadi Al Johfa, Wadi Al Hakak, and Wadi Khurieba. The percentage of polymorphic loci PPL% were ranged between 60% and 32% and a considerable inbreeding value (F = 0.942). Elevated genetic differentiation among the populations and a low gene migration suggest isolation among S. persica populations. Several recommendations for the protection of the existing populations, including seed preservation, restoration of highly endangered sites, and management of cutting and grazing activities.

摘要

Salvadora persica L.是分布在沙漠和亚热带地区的药用植物。在拉比格（Rabigh），这种植物的极少数持久种群遭受过度砍伐，过度放牧以及最近人类居住。波斯菊的保护需要对其遗传多样性和遗传分化进行评估。我们应用了40个简单序列重复（SSR）引物，已鉴定出22个多态位点。PIC值在0.858至0.967之间变化。从拉比格的四个地点采样了240种植物种质：Wadi EL Khaneg，Wadi Al Johfa，Wadi Al Hakak和Wadi Khurieba。多态位点PPL％的百分比在60％至32％之间，并且具有相当的近交值（F ＝ 0.942）。种群之间遗传分化程度的提高和低基因迁移表明，波斯菊种群之间存在隔离。关于保护现有种群的几项建议，包括种子保存，高度濒危地区的恢复以及割草和放牧活动的管理。