A reliable and sensitive micelle based liquid chromatography (MLC) was developed aiming to simultaneously estimate three binary antibiotic mixtures namely: cefixime (CFX) in combination with either linezolid (LNZ) (mixture Ι) or levofloxacin hemihydrate (LEV) (mixture II), and nifuroxazide (NX) with drotaverine HCl (DV) (mixture IΙI). All mixtures were separated using a micellar mobile phase prepared in 0.02 M phosphoric acid and consisting of an anionic surfactant; sodium dodecyl sulphate, triethylamine (0.3%), n-propanol, adjusted to pH 2.5 (for mixtures Ι and ΙΙ) or pH 5 for mixture ΙΙΙ with detection wavelengths of 254, 295, and 210 nm for the three mixtures respectively. Separation of the first two mixtures was conducted using a C18 column, while the last mixture was separated using a C18 monolithic column. Drugs in mixture I were rectilinear at (1.0–320.0 µg/mL) and (0.5–320.0 µg/mL) for CFX and LNZ, respectively with corresponding detection limits of 0.3269 and 0.0512 µg/mL and quantitation limits of 0.9906 and 0.1552 µg/mL, while pharmaceutical agents in mixture II showed linearity over the ranges of (0.125–80.0 µg/mL) and (0.25–160.0 µg/mL) for CFX and LEV, respectively with corresponding detection limits of 0.0154 and 0.026 µg/mL and quantitation limits of 0.0465 and 0.0789 µg/mL. Whereas linearity ranges of the analytes in mixture ΙΙΙ were 0.5–400.0 µg/mL and 2.0–400.0 µg/mL for NX and DV respectively with corresponding detection limits of 0.1822 and 0.2631 µg/mL and quantitation limits of 0.5522 and 0.7974 µg/mL. The proposed method was thoroughly validated; besides it was applied to estimate the concerned analytes in their dosage forms. The results obtained from the developed method and the reference methods were in accordance. The proposed method was further extended to determine drugs of mixtures Ι and ΙΙ in spiked human plasma without any extraction steps with high recovery percentages.

开发了一种可靠，敏感的基于胶束的液相色谱（MLC），旨在同时评估三种二元抗生素混合物，即头孢克肟（CFX）与利奈唑胺（LNZ）（混合物Ⅰ）或左氧氟沙星半水合物（LEV）（混合物II）的组合，和尼呋沙嗪（NX）与盐酸屈拉维林（DV）（混合物II）。使用在0.02 M磷酸中制备的，由阴离子表面活性剂组成的胶束流动相分离所有混合物。将十二烷基硫酸钠，三乙胺（0.3％），正丙醇调节至pH 2.5（对于混合物II和III）或对于混合物III调节至pH 5，分别对三种混合物的检测波长为254、295和210 nm。前两个混合物使用C18色谱柱进行分离，而最后一个混合物使用C18整体色谱柱进行分离。混合物I中的CFX和LNZ的线性分别为（1.0–320.0 µg / mL）和（0.5–320.0 µg / mL），分别的检出限为0.3269和0.0512 µg / mL，定量限为0.9906和0.1552 µg / mL毫升，而混合物II中的药剂对CFX和LEV的线性范围分别为（0.125–80.0 µg / mL）和（0.25–160.0 µg / mL），分别具有0.0154和0.026 µg / mL的检出限和定量限值为0.0465和0.0789 µg / mL。而混合物Ⅲ中分析物的线性范围分别是NX和DV为0.5–400.0 µg / mL和2.0–400.0 µg / mL，相应的检出限为0.1822和0.2631 µg / mL，定量限为0.5522和0.7974 µg / mL。所提出的方法得到了充分的验证；此外，它还用于估算剂型中有关的分析物。从开发的方法和参考方法获得的结果是一致的。所提出的方法被进一步扩展以测定加标的人血浆中混合物I和II的药物，而无需任何具有高回收率的提取步骤。