Contamination of multiple antineoplastic drugs (ADs) on work surfaces presents an exposure concern for health care workers. Surface wipe sampling is a recognized method to evaluate the degree of contamination present. Our research team has previously reported on wipe sampling and analytical methods to simultaneously detect 10 commonly used ADs from a single wipe. Our objectives here were: to field test a protocol consisting of the wipe sampling method and an accompanying wipe sample collection tool kit and confirm this protocol can be effectively used by health care workers to assess drug contamination levels in their facilities; and, to confirm the potential for simultaneous exposure to multiple antineoplastic drugs. Three facilities within one health authority in British Columbia, Canada participated in this field study. In collaboration with the site health and safety advisors, up to 25 surfaces within each facility were considered for sampling. Collected wipe samples were analyzed using HPLC-MS/MS to quantify the 10 analyte, resulting in 750 potential analyses. Following the sampling, each of the three facilities’ safety advisors provided feedback regarding the usability of the protocols. Among the 72 wipe samples actually collected (or 720 analyses conducted), detectable levels and simultaneous contamination of work surfaces of five of the 10 analytes were found at all three participating sites: 5-fluorouracil, cyclophosphamide, vincristine, paclitaxel, and methotrexate; (range < LoD to 33.0 ng/cm²) with 5-fluorouracil having the highest concentration in every instance. Drug contamination was found on a variety of different work surfaces in pharmacies and patient care areas among all three sites. Users of the sampling protocols were generally satisfied with the wipe sample collection toolkit with some minor suggestions for improvement. Our findings support the hypothesis that health care workers may be simultaneously at risk of exposure to several ADs. Our toolkit was found to be user-friendly and manageable by those who were not experienced in collecting wipe samples to monitor contamination of ADs on the work surfaces in their facilities.

工作表面上多种抗肿瘤药（AD）的污染为医护人员带来了暴露隐患。表面擦拭采样是一种公认​​的评估污染程度的方法。我们的研究团队此前曾报告过擦拭采样和分析方法，可从一次擦拭中同时检测10个常用AD。我们的目标是：对由擦拭物采样方法和随附的擦拭物样本收集工具套件组成的协议进行现场测试，并确认医护人员可以有效地使用此协议来评估其设施中的药物污染水平；并确认同时暴露于多种抗肿瘤药的潜力。加拿大不列颠哥伦比亚省一个卫生机构内的三个机构参加了这项现场研究。在与现场健康和安全顾问的协作下，每个设施中最多考虑25个表面进行采样。使用HPLC-MS / MS分析收集的擦拭样品，以定量分析10种分析物，从而进行750项潜在分析。采样后，三个机构的安全顾问均提供了有关协议可用性的反馈。在实际收集的72个擦拭样品中（或进行了720次分析），在所有三个参与位置发现了10种分析物中的5种的可检测水平并同时污染了工作表面：5-氟尿嘧啶，环磷酰胺，长春新碱，紫杉醇和甲氨蝶呤；（范围<LoD至33.0 ng / cm 使用HPLC-MS / MS分析收集的擦拭样品，以定量分析10种分析物，从而进行750项潜在分析。采样后，三个机构的安全顾问均提供了有关协议可用性的反馈。在实际收集的72个擦拭样品中（或进行了720次分析），在所有三个参与位置发现了10种分析物中的5种的可检测水平并同时污染了工作表面：5-氟尿嘧啶，环磷酰胺，长春新碱，紫杉醇和甲氨蝶呤；（范围<LoD至33.0 ng / cm 使用HPLC-MS / MS分析收集的擦拭样品，以定量分析10种分析物，从而进行750项潜在分析。采样后，三个机构的安全顾问均提供了有关协议可用性的反馈。在实际收集的72个擦拭样品中（或进行了720次分析），在所有三个参与位置发现了10种分析物中的5种的可检测水平并同时污染了工作表面：5-氟尿嘧啶，环磷酰胺，长春新碱，紫杉醇和甲氨蝶呤；（范围<LoD至33.0 ng / cm 在所有三个参与地点发现了10种分析物中的5种的可检测水平并同时污染了工作表面：5-氟尿嘧啶，环磷酰胺，长春新碱，紫杉醇和甲氨蝶呤；（范围<LoD至33.0 ng / cm 在所有三个参与地点发现了10种分析物中的5种的可检测水平并同时污染工作表面：5-氟尿嘧啶，环磷酰胺，长春新碱，紫杉醇和甲氨蝶呤；（范围<LoD至33.0 ng / cm²）在每种情况下具有最高浓度的5-氟尿嘧啶。在这三个地点的药房和患者护理区的各种不同工作面上都发现了药物污染。采样协议的用户通常对擦除样本收集工具包感到满意，并提出了一些小的改进建议。我们的研究结果支持以下假设：医护人员可能同时处于暴露于数个AD的风险中。我们的工具包被那些没有经验的人收集和使用，他们没有经验来收集擦拭样品来监控其工作表面上的AD污染。