Abstract Microwave-assisted hydrodistillation and conventional hydrodistillation (MAHD and HD) were used for a comparative study of extraction Callistemon citrinus Skeels leaf essential oils. The time required for a MAHD method is 75 minutes that for HD method is 3.5 hours, so MAHD requires lesser time. The increases in yield of extracted oil by MAHD (0.84%) is found compared oil extracted by HD (0.6%). Essential oils were examined by GC–MS. MAHD extracted essential oils were wealthier in oxygenated molecules. The main constituent was 1, 8-cineole, followed by α-pinene in the case of MAHD. Percentage of the major component increases from 80% to 90% as compared to HD. Their antibacterial activity was examined on ‘Gram-negative’ and ‘Gram-positive bacteria’. In the case of both bacteria, MAHD extracted oil shows better activity than HD extracted one. Antioxidant action was studied by using 2, 2-diphenyl-1-picrylhydrazyl (DPPH•) radical scavenging and β-carotene/linoleic acid assay it is shown that MAHD extract has better activity than HD extract. Surface morphology of Callistemon leaves was studied with scanning electronic microscopy (SEM) which reveals that the MAHD leaves surface undergo major damage to the conventional HD.

中文翻译：

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常规和微波辅助加氢蒸馏法获得的柑橘柑橘（瓶刷）精油的化学成分、抗氧化和抗菌活性比较

摘要 微波辅助加水蒸馏和常规加水蒸馏（MAHD 和 HD）用于提取柑橘香柠檬叶精油的比较研究。MAHD 方法所需的时间为 75 分钟，而 HD 方法为 3.5 小时，因此 MAHD 需要的时间更少。发现与 HD 提取的油 (0.6%) 相比，MAHD 提取的油的产量增加 (0.84%)。通过 GC-MS 检测精油。MAHD 提取的精油在含氧分子中更丰富。在 MAHD 的情况下，主要成分是 1, 8-桉树脑，其​​次是 α-蒎烯。与 HD 相比，主要成分的百分比从 80% 增加到 90%。他们对“革兰氏阴性菌”和“革兰氏阳性菌”的抗菌活性进行了检查。在这两种细菌的​​情况下，MAHD 提取的油比 HD 提取的油显示出更好的活性。通过使用 2, 2-diphenyl-1-picrylhydrazyl (DPPH•) 自由基清除和 β-胡萝卜素/亚油酸测定来研究抗氧化作用，结果表明 MAHD 提取物比 HD 提取物具有更好的活性。用扫描电子显微镜 (SEM) 研究了 Callistemon 叶子的表面形态，结果表明 MAHD 叶子表面对传统 HD 造成了重大损害。