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New polymorphic microsatellite markers for Sarcandra glabra (Chloranthaceae), an evergreen broad-leaved shrub endangered in South Korea
Journal of Forest Research ( IF 1.3 ) Pub Date : 2020-06-22 , DOI: 10.1080/13416979.2020.1783052
Eun-Kyeong Han 1 , Won-Bum Cho 2 , Goya Choi 3 , Sungyu Yang 3 , Hyeok-Jae Choi 4 , Gwan-Pil Song 5 , Jung-Hyun Lee 2
Affiliation  

Microsatellite markers were newly isolated for Sarcandra glabra (Chloranthaceae), an ecologically important species in the evergreen broad-leaved forests of East Asia. On the Korean Peninsula, S. glabra is endangered, being extremely restricted to a few habitats on Jeju Island, which is the northern edge of their distribution range with evolutionary potential. Recently, populations have been rapidly declining internationally, increasing the urgency for the conservation of Jeju populations. For long-term conservation, we designed 24 polymorphic microsatellite markers in three multiplex sets. S. glabra frequently breed through vegetative reproduction. Therefore, the ability to detect clones was also tested. From two natural populations, the number of alleles per locus ranged from 2 to 14 (average, 4.292). Values for expected heterozygosity ranged from 0.000 to 0.891 (mean, 0.263). As a result of the genotype accumulation curve, the complete recognition (100%) of all multilocus genotypes can be achieved using only 21 loci, indicating that markers showed a high ability to accurately identify individuals in natural populations. These markers will be beneficial for establishing suitable conservation strategies for S. glabra in East Asia as well as Jeju Island.



中文翻译:

Sarcandra glabra(Chloranthaceae)的一种新的多态微卫星标记,一种在韩国濒临灭绝的常绿阔叶灌木

为东亚常绿阔叶林中具有重要生态意义的物种Sarcandra glabra(Chloranthaceae)新分离了微卫星标记。在朝鲜半岛,S。glabra濒临灭绝,极度局限于济州岛的一些栖息地,济州岛是其分布范围的北部边缘,具有进化潜力。近来,国际上人口迅速减少,这增加了保护济州人口的紧迫性。为了长期保存,我们在三个多重集合中设计了24个多态微卫星标记。格拉伯链球菌通常通过无性繁殖繁殖。因此,还测试了检测克隆的能力。在两个自然种群中,每个基因座的等位基因数量范围为2到14(平均值为4.292)。预期杂合度的值在0.000到0.891之间(平均值为0.263)。基因型累积曲线的结果是,仅使用21个基因座就可以实现所有多基因座基因型的完全识别(100%),这表明标记物显示出高准确度地识别自然种群中个体的能力。这些标记物将有利于为东亚和济州岛的S. glabra建立合适的保护策略。

更新日期:2020-06-22
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