Abstract

Bioactivity modification helps polymethylmethacrylate (PMMA) bone cement to reinforce its interfacial adhesion to bone tissues through the chemical bonding of apatite. Since Si-OH groups combined with Ca²⁺ ions have succeeded in inducing apatite formation, more combinations of functional groups and active ions are being explored. In this study, Bis[2-(methacryloyloxy)ethyl] phosphate (B2meP) containing phosphate (=PO₄H) groups and Ca(CH₃COO)₂ supplying Ca²⁺ ion were adopted to investigate the feasibility of equipping PMMA bone cement with apatite-forming ability in vitro, more effects under designed contents on setting behavior, injectability, contact angle, cytotoxicity and mechanical strength were also investigated. Results showed B2meP copolymerized with MMA and became one section of PMMA chains, surface = PO₄H groups and released Ca²⁺ ions pushed spherical apatite individuals nucleating and agglomerating into layer horizontally, Increasing B2meP content lowered the contact angle and the peak temperature, enhanced the cell viability of MC3T3-E1, but prolonged apatite forming period. Injectability rate performed a similar trend to setting time. Lower adding content and deposited apatite layer contributed to reduce the strength loss in soaking. Taking biological performance and other properties into balance, cement added with B2meP of 10 wt% in MMA and Ca(CH₃COO)₂ of 20 wt% in PMMA performed better.

摘要

生物活性改性有助于聚甲基丙烯酸甲酯 (PMMA) 骨水泥通过磷灰石的化学结合增强其与骨组织的界面粘附。由于 Si-OH 基团与 Ca ²⁺离子结合已成功诱导磷灰石形成，因此正在探索更多官能团和活性离子的组合。本研究采用含磷酸根（=PO ₄ H）基团和提供Ca ²⁺离子的Ca(CH ₃ COO) _{2 的}双[2-(甲基丙烯酰氧基)乙基]磷酸酯(B2meP)来研究装备PMMA骨水泥的可行性具有体外磷灰石形成能力，还研究了在设计内容下对凝固行为、可注射性、接触角、细胞毒性和机械强度的更多影响。结果表明 B2meP 与 MMA 共聚成为 PMMA 链的一部分，表面 = PO ₄ H 基团并释放 Ca ²⁺离子推动球形磷灰石个体水平成核和团聚，增加B2meP含量降低了接触角和峰值温度，提高了MC3T3-E1的细胞活力，但延长了磷灰石的形成时间。注射率表现出与凝固时间相似的趋势。较低的添加量和沉积的磷灰石层有助于减少浸泡时的强度损失。平衡生物性能和其他性能，在MMA中添加10wt%的B2meP和在PMMA中添加20wt%的Ca(CH ₃ COO) ₂的水泥表现更好。