Abstract

Surface modification of titanium implants by siRNA is quite efficient for improving implant osseointegration. Loading siRNA onto their surface is a crucial factor for siRNA-functionalized implants to realize their biological function. Direct binding of siRNA to implants has low siRNA binding and releasing rate, so usually it needs to be mediated by vectors. Polymeric, nonmaterial-mediated and lipid-based vectors are types of non-viral vectors which are commonly used for delivering siRNA. Three major methods of loading process, namely simple physical adsorption, layer-by-layer assembly and electrodeposition, are also summarized. A brief introduction, the basic principle and the general procedure of each method are included. The loading efficiency, which can be measured both qualitatively and quantitatively, together with gene knockdown efficiency, cytotoxicity assay and osteogenesis of the three methods are compared. A good many applications in osteogenesis have also been described in this review.

摘要

通过 siRNA 对钛种植体进行表面改性对于改善种植体骨整合非常有效。将siRNA加载到其表面是siRNA功能化植入物实现其生物学功能的关键因素。siRNA与植入物的直接结合，siRNA的结合率和释放率较低，通常需要通过载体介导。聚合的、非材料介导的和基于脂质的载体是常用于递送 siRNA 的非病毒载体类型。还总结了三种主要的负载过程方法，即简单的物理吸附、逐层组装和电沉积。包括简要介绍、基本原理和每种方法的一般过程。加载效率，可以定性和定量测量，以及基因敲低效率，比较了三种方法的细胞毒性测定和成骨。本综述还描述了在成骨中的许多应用。