Abstract

Cellulose, a polysaccharide of β (1–4) linked D-glucose units, is a cheap, eco-friendly and most abundant natural polymer on this planet. Among various cellulosic materials, cotton cellulose is readily available, lignin-free, FDA approved, and widely used in the medical field because of its higher degree of biocompatibility and non-cytotoxic nature. Though cotton cellulose showed essential material properties for scaffold design, the least priority had been given to this material. The present study aimed at exploring the fabrication of scaffold using cotton microfibers for bone tissue engineering application. The study also aimed at improving the mechanical, physio-chemical and osteogenic properties of the microfibrous scaffold by crosslinking with citric acid and further modified with gelatin. FTIR indicated some interactions between cellulose, citric acid and gelatin within the scaffolds, while XRD results demonstrated the crystalline nature of scaffolds. Porosity and swelling studies demonstrated that all scaffolds are hydrophilic and porous. The microporous interconnected network of scaffolds was confirmed by FESEM. FESEM micrographs and MTT assay confirmed that all scaffolds were nontoxic to MG 63. Based on findings, it was concluded that gelatin coated cotton cellulose microfibers crosslinked with citric acid scaffold would be a potential template for bone tissue engineering.

摘要

纤维素是一种由 β (1-4) 连接的 D-葡萄糖单元组成的多糖，是地球上廉价、环保和最丰富的天然聚合物。在各种纤维素材料中，棉纤维素因其较高的生物相容性和无细胞毒性而易于获得、不含木质素、经 FDA 批准并广泛用于医学领域。尽管棉纤维素显示出支架设计的基本材料特性，但这种材料的优先级最低。本研究旨在探索使用棉微纤维制造用于骨组织工程应用的支架。该研究还旨在通过与柠檬酸交联并用明胶进一步改性来改善微纤维支架的机械、理化和成骨特性。FTIR 表明纤维素之间存在一些相互作用，支架内的柠檬酸和明胶，而 XRD 结果证明了支架的结晶性质。孔隙率和溶胀研究表明，所有支架都是亲水性和多孔性的。FESEM证实了支架的微孔互连网络。FESEM 显微照片和 MTT 测定证实，所有支架对 MG 63 均无毒。根据研究结果，得出结论，与柠檬酸支架交联的明胶涂层棉纤维素微纤维将是骨组织工程的潜在模板。