Abstract

Microbial adhesion reduction as well as platelet compatibility improvement have been suggested as the key requirements for developing blood-contacting synthetic biomaterials. Surface grafting of hydrophilic polyethylene glycol chains or alkyl chains with zwitterionic terminal ends has been proposed for reducing microbial or platelet adhesion. Nonetheless, none has been reported to incorporate both polyethylene glycol and zwitterionic terminal functionality on the same surface-grafted alkyl chain. In this investigation, a novel surface modification scheme was reported for grafting zwitterionic alkyl chains with or without polyethylene glycol as the spacer. It was noted the bacterial adhesion reduction capability on the zwitterionic modified surface was dependent upon the use of polyethylene glycol spacer or not and the strain of microbe tested. Besides, the zwitterionic modified ones all showed greater antimicrobial adhesion capability than the surface modified with polyethylene glycol alone. On the other hand, significantly reduced platelet adhesion and activation were found, but with no statistical differences noted among the polyethylene glycol-modified surface and zwitterionic ones, with or without polyethylene glycol spacer. These suggested that the use of polyethylene glycol spacer on the zwitterionic terminated surface could further enhance the antimicrobial adhesion against gram-negative bacterial while still keeping its platelet compatibility.

摘要

减少微生物粘附以及改善血小板相容性已被认为是开发与血液接触的合成生物材料的关键要求。亲水性聚乙二醇链或具有两性离子末端的烷基链的表面接枝已被提议用于减少微生物或血小板粘附。尽管如此，还没有报道将聚乙二醇和两性离子末端官能团结合在同一表面接枝的烷基链上。在这项研究中，报道了一种新的表面改性方案，用于接枝有或没有聚乙二醇作为间隔基的两性离子烷基链。注意到两性离子改性表面上的细菌粘附减少能力取决于是否使用聚乙二醇间隔物以及测试的微生物菌株。此外，两性离子改性的表面均显示出比单独用聚乙二醇改性的表面更强的抗菌粘附能力。另一方面，发现血小板粘附和活化显着降低，但在聚乙二醇改性表面和两性离子表面之间没有发现统计学差异，有或没有聚乙二醇间隔物。这些表明在两性离子终止表面上使用聚乙二醇间隔物可以进一步增强对革兰氏阴性细菌的抗菌粘附，同时仍保持其血小板相容性。但在聚乙二醇改性表面和两性离子表面之间没有发现统计学差异，有或没有聚乙二醇间隔物。这些表明在两性离子终止表面上使用聚乙二醇间隔物可以进一步增强对革兰氏阴性细菌的抗菌粘附，同时仍保持其血小板相容性。但在聚乙二醇改性表面和两性离子表面之间没有发现统计学差异，有或没有聚乙二醇间隔物。这些表明在两性离子终止表面上使用聚乙二醇间隔物可以进一步增强对革兰氏阴性细菌的抗菌粘附，同时仍保持其血小板相容性。