The distribution of the honey bee lineages in North Lebanon and Akkar governorates was analyzed using the mitochondrial DNA (MtDNA) test through the amplification of the COI-COII region by polymerase chain reaction followed by DraI enzyme restriction analysis. A total of 95 samples were collected from 32 different locations, in addition to two reference samples. MtDNA analysis showed that 72% of the studied samples belong to the native subspecies A. m. syriaca corresponding to the Z subgroup of lineage A, mainly found in regions of North Lebanon and northern part of Akkar. Samples belonging to the C lineage were more distributed in the inner, eastern and southern regions of Akkar. The regions where the Z subgroup still prevailed could be considered as candidate reserve areas for the local subspecies.

中文翻译：

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使用线粒体 DNA 测试对黎巴嫩蜜蜂亚种进行遗传筛查

使用线粒体 DNA (MtDNA) 测试，通过聚合酶链反应扩增 COI-COII 区域，然后进行 DraI 酶限制分析，分析了北黎巴嫩和阿卡省蜜蜂谱系的分布。除了两个参考样品外，总共从 32 个不同地点收集了 95 个样品。MtDNA 分析表明，72% 的研究样本属于本地亚种 A. m. syriaca 对应于谱系 A 的 Z 亚群，主要发现于黎巴嫩北部和阿卡北部地区。属于 C 谱系的样品更多地分布在阿卡的内部、东部和南部地区。Z亚群仍然盛行的地区可作为当地亚种的候选保护区。