Propolis has been used in ethnobotany and traditional practices and is currently considered a source of phytotherapeutics. The aim of this study was to develop an antibiotic and antioxidant hydrogel containing Argentine propolis extract and evaluate its stability for one year in different conditions. A fingerprint by HPLC-DAD of propolis extract showed the presence of caffeic, coumaric, ferulic and cinnamic acids, caffeic acid phenethyl ester (CAPE) and flavonoids such as pinobanksin, apigenin, kaempferol, pinocembrin, chrysin, and galangin. The minimal inhibitory concentration (MIC) and minimal bactericidal concentration (MBC) of propolis extract on clinical isolates (Gram-negative and Gram-positive) from the skin and soft tissue infections were between 25–100 μgGAE/ml and 50–200 μgGAE/ml, respectively. The concentration of propolis extract required to scavenge 50% of free radicals was 2 μgGAE/ml. The hydrogel with extract kept its chemical, physical and microbiological stability, and its biological properties over 1 year of storage, mainly at 4 °C and at room temperature and light-protected. In addition, topical formulation showed good results in the release assay of active principle by using Franz’s cell. These studies suggest that the topical formulation could be used as an antioxidant and antibacterial topical apitherapeutic product.

中文翻译：

---

含有阿根廷蜂胶的水醇凝胶：抗菌和抗氧化活性的潜力、稳定性评估和体外酚类释放

蜂胶已用于民族植物学和传统实践，目前被认为是植物疗法的来源。本研究的目的是开发一种含有阿根廷蜂胶提取物的抗生素和抗氧化水凝胶，并评估其在不同条件下一年的稳定性。蜂胶提取物的 HPLC-DAD 指纹图谱显示存在咖啡酸、香豆酸、阿魏酸和肉桂酸、咖啡酸苯乙酯 (CAPE) 和黄酮类化合物，如松香素、芹菜素、山奈酚、松香素、白杨素和高良姜素。蜂胶提取物对皮肤和软组织感染的临床分离株（革兰氏阴性和革兰氏阳性）的最小抑菌浓度（MIC）和最小杀菌浓度（MBC）在 25-100 μgGAE/ml 和 50-200 μgGAE/毫升，分别。清除 50% 自由基所需的蜂胶提取物浓度为 2 μgGAE/ml。含有提取物的水凝胶保持其化学、物理和微生物稳定性，并在 1 年以上的储存中保持其生物学特性，主要是在 4°C 和室温和避光条件下。此外，外用制剂在使用Franz's细胞的活性成分释放试验中显示出良好的结果。这些研究表明，外用制剂可用作抗氧化剂和抗菌外用药物治疗产品。外用制剂在使用 Franz 细胞的活性成分释放试验中显示出良好的结果。这些研究表明，外用制剂可用作抗氧化剂和抗菌外用药物治疗产品。外用制剂在使用 Franz 细胞的活性成分释放试验中显示出良好的结果。这些研究表明，外用制剂可用作抗氧化剂和抗菌外用药物治疗产品。