Major royal jelly proteins (MRJPs) are deemed to the most characteristic and abundant constituents in royal jelly. The present paper establishes a sequential process to purify the natural MRJP 1–3 by three different chromatographic procedures. Five individual proteins were obtained, including MRJP 1 oligomer 1, MRJP 1 oligomer 2, MRJP 1 monomer, MRJP 2 and MRJP 3s (contain three MRJP 3 variants) with a purity of 89.88%, 67.79%, 99.92%, 99.41%, and 99.97%, respectively. This is the first report that MRJP 1 mainly possesses three oligomers: a 228 kDa MRJP 1 oligomer 1, a 408 kDa MRJP 1 oligomer 2 and a 639 kDa MRJP 1 oligomer 3. We infer that MRJP 1 oligomer 3 (639 kDa) may be assembled by MRJP 1 oligomer 1 (228 kDa) and MRJP 1 oligomer 2 (408 kDa). The secondary structure of the purified MRJP 1–3 consisted predominantly of β-sheets and random coil in the native conformation. Our results will contribute to the physiological and physicochemical function of the purified individual MRJPs.

中文翻译：

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单个主要蜂王浆蛋白1-3的纯化及特性

主要蜂王浆蛋白 (MRJPs) 被认为是蜂王浆中最具特征和最丰富的成分。本论文建立了通过三种不同的色谱程序纯化天然 MRJP 1-3 的顺序过程。获得了五种单独的蛋白质，包括 MRJP 1 oligomer 1、MRJP 1 oligomer 2、MRJP 1 单体、MRJP 2 和 MRJP 3s（包含三个 MRJP 3 变体），纯度分别为 89.88%、67.79%、99.92%、99.41% 和分别为 99.97%。这是首次报道 MRJP 1 主要拥有三种低聚物：228 kDa MRJP 1 低聚物 1、408 kDa MRJP 1 低聚物 2 和 639 kDa MRJP 1 低聚物 3。我们推断 MRJP 1 低聚物 3 (639 kDa)由 MRJP 1 低聚物 1 (228 kDa) 和 MRJP 1 低聚物 2 (408 kDa) 组装而成。纯化的 MRJP 1-3 的二级结构主要由天然构象的 β-折叠和无规卷曲组成。我们的结果将有助于纯化单个 MRJP 的生理和物理化学功能。