Abstract

As a poly(D-glucosamine), chitosan possesses the ability to chelate bioactive metal ions such as copper and zinc. In this work, metal ion containing microspheres were formed through complexation interactions between glucosamine unit of chitosan and copper and zinc ions, respectively. The successful production of chitosan‒metal ion microspheres was done by high-voltage electrostatic system. The produced microspheres showed a narrow size distribution with average size range of 100–240 µm. The cytotoxicity evaluation of direct cells‒materials contact indicated no toxicity of chitosan–Zn²⁺ microspheres, while chitosan‒Cu²⁺ microspheres containing higher c(Cu²⁺) were cytotoxic for Hek293 cells during 3 days of culture.

摘要

作为聚（D-葡糖胺），壳聚糖具有螯合生物活性金属离子如铜和锌的能力。在这项工作中，通过壳聚糖的氨基葡萄糖单元与铜和锌离子之间的络合相互作用分别形成了含有金属离子的微球。通过高压静电系统成功制备壳聚糖-金属离子微球。生产的微球显示出窄的尺寸分布，平均尺寸范围为 100-240 µm。细胞-材料直接接触的细胞毒性评估表明壳聚糖-Zn ²⁺微球没有毒性，而含有较高c（Cu ²⁺）的壳聚糖-Cu ²⁺微球在培养3天期间对Hek293细胞具有细胞毒性。