Abstract

Some of the trauma patients (about 3%) suffer from peripheral nerve injuries and unfortunately, it is still a significant clinical problem to regenerate and functional recovery of them. These days, peripheral nerve damages have been treated with various methods and materials. The goal of the present study is to evaluate the effect of various amounts of 4-Methylcatechol (4-MC) on peripheral nerve regeneration. For this target, alginate/chitosan hydrogel was used to deliver different percentages (0.1%, 1%, and 10% (w/v)) of 4-MC to the sciatic nerve-injured sites in a rat model. Before that, the properties of hydrogels were assessed and after injecting, different functional analyses were used to evaluate the efficacy of fabricated hydrogels on peripheral nerve regeneration. Our results exhibited that the pore size of hydrogels was appropriate for cell proliferation (26–42 µm). Biodegradability of hydrogels was about 70% after 7 days and hemocompatibility of hydrogels was also confirmed. The MTT assay showed that the hydrogels not only do not have toxicity effects but also can improve the proliferation rate of PC12 cells. The functional results showed that 4-MC especially the group contained 10% of 4-MC enhance sciatic nerve regeneration. It could be concluded that fabricated hydrogels can use as suitable materials for peripheral nerve regeneration.

摘要

一些外伤患者（约 3%）患有周围神经损伤，不幸的是，他们的再生和功能恢复仍然是一个重大的临床问题。目前，周围神经损伤已用各种方法和材料进行治疗。本研究的目的是评估不同量的 4-甲基儿茶酚 (4-MC) 对周围神经再生的影响。对于这个目标，海藻酸盐/壳聚糖水凝胶用于将不同百分比（0.1%、1% 和 10% (w/v)）的 4-MC 递送到大鼠模型中的坐骨神经损伤部位。在此之前，评估了水凝胶的特性，并在注射后，使用不同的功能分析来评估制造的水凝胶对周围神经再生的功效。我们的结果表明，水凝胶的孔径适合细胞增殖（26-42 µm）。7 天后水凝胶的生物降解性约为 70%，并且还证实了水凝胶的血液相容性。MTT实验表明，该水凝胶不仅没有毒性作用，而且可以提高PC12细胞的增殖率。功能结果表明4-MC尤其是含10%4-MC的组有促进坐骨神经再生的作用。可以得出结论，制造的水凝胶可以用作外周神经再生的合适材料。MTT实验表明，该水凝胶不仅没有毒性作用，而且可以提高PC12细胞的增殖率。功能结果表明4-MC尤其是含10%4-MC的组有促进坐骨神经再生的作用。可以得出结论，制造的水凝胶可以用作外周神经再生的合适材料。MTT实验表明，该水凝胶不仅没有毒性作用，而且可以提高PC12细胞的增殖率。功能结果表明4-MC尤其是含10%4-MC的组有促进坐骨神经再生的作用。可以得出结论，制造的水凝胶可以用作外周神经再生的合适材料。