Abstract

For blood vessel regeneration, rapid endothelialization is essential by the effective modulation of vascular endothelial cells (VECs). Herein, microRNA-126 was loaded in electrospun membranes of poly(ethylene glycol)-b-poly(L-lactide-co-ε-caprolactone) combined with REDV peptide to promote VEC growth. The prepared electrospun membranes with suitable mechanical properties in the wet state could accelerate microRNA-126 release to 82.5 ± 4.0% in 56 days. VECs cultured on the electrospun membranes exhibited enhanced adhesion and proliferation with elongating and aligning morphology to the flow direction under shear stress compared with the static culture. The electrospun membranes containing REDV and microRNA-126 could have great potential in applications of bioactive vascular grafts.

摘要

对于血管再生，通过有效调节血管内皮细胞 (VEC)，快速内皮化是必不可少的。在此，微小RNA-126装载在静电膜的聚（乙二醇） - b -聚（L丙交酯-共- ε-己内酯）与 REDV 肽结合以促进 VEC 生长。制备的电纺膜在潮湿状态下具有合适的机械性能，可以在 56 天内将 microRNA-126 的释放加速至 82.5 ± 4.0%。与静态培养相比，在电纺膜上培养的 VEC 表现出增强的粘附和增殖，在剪切应力下具有伸长和与流动方向对齐的形态。含有 REDV 和 microRNA-126 的电纺膜在生物活性血管移植物的应用中具有巨大的潜力。