Abstract

The adequacy of biomaterials is essential to generate scaffolds suitable for tissue engineering studies. The electrospinning technique is a resourceful, quick and cheap way to obtain polycaprolactone (PCL) scaffolds with an architecture with porosity and adequate properties to ensure the survival of cells. However, some obstacles need to be overcome, such as the hydrophilicity of this polymer. In this study, we produced electrospun PCL nanofibrous mats and submitted them to aminolysis with three different diamines in order to generate hydrophilic cell-friendly scaffolds. The nanofibrous mats were characterized by their morphology, thermal and mechanical properties and, lastly, seeded with Human Adipose-Derived Stem Cells (hADSC) to observe the influence of the introduced amino groups in the polymeric matrix. The obtained results suggest a successful insertion of amino groups in the polymeric matrix, without being too detrimental to its morphology and intrinsic properties. Furthermore, the aminolyzed mats displayed a qualitative increase of cells attached to the nanofibrous mats.

摘要

生物材料的充足性对于生成适合组织工程研究的支架至关重要。静电纺丝技术是获得聚己内酯 (PCL) 支架的一种资源丰富、快速且廉价的方法，其结构具有多孔性和足够的特性，可确保细胞的存活。然而，需要克服一些障碍，例如这种聚合物的亲水性。在这项研究中，我们生产了电纺 PCL 纳米纤维垫，并用三种不同的二胺将它们进行氨解，以生成亲水的细胞友好支架。纳米纤维垫的特征在于它们的形态、热和机械性能，最后接种人类脂肪干细胞 (hADSC) 以观察聚合物基质中引入的氨基的影响。获得的结果表明氨基在聚合物基质中的成功插入，而对其形态和内在特性没有太大的损害。此外，氨解垫显示出附着在纳米纤维垫上的细胞数量增加。