Abstract

Poly(Ɛ-caprolactone) (PCL) electrosprayed nanoparticles (NPs) containing Amphotericin B (AMB) were prepared for the treatment of leishmaniasis. Two variations of PCL nanoparticles with positive surface charges, which can enhance cell uptake and restrict agglomeration, were produced: (1) NPs containing stearylamine and (2) core-shell nanoparticles, with chitosan in the shell and PCL and AMB in the core. The results proved the viability of producing positively charged PCL nanoparticles with AMB, with diameters <350 nm. The encapsulation efficiency of the drug was higher than 75%. The electrosprayed nanoparticles containing AMB were much less cytotoxic to cells than that of pure AMB.

摘要

制备含有两性霉素 B (AMB) 的聚 (Ɛ-己内酯) (PCL) 电喷雾纳米粒子 (NPs) 用于治疗利什曼病。产生了两种具有正表面电荷的 PCL 纳米粒子，它们可以增强细胞摄取并限制团聚：（1）含有硬脂胺的纳米粒子和（2）核壳纳米粒子，壳中含有壳聚糖，核中含有 PCL 和 AMB。结果证明了用 AMB 生产带正电荷的 PCL 纳米颗粒的可行性，直径 <350 nm。药物的包封率高于75%。与纯 AMB 相比，含有 AMB 的电喷雾纳米颗粒对细胞的细胞毒性要小得多。