Abstract In this paper, a series of porous nanohydroxyapatite/silk fibroin/chitosan (nHA/SF/CTS) scaffolds were successfully prepared using the freeze-drying method. The biomaterials were characterized by attenuated total reflection Fourier transform infrared spectroscopy, and mechanical testing and thermogravimetric analysis. Moreover, studies of porosity, pore size, swelling properties and in vitro degradation test were performed. Research has proved that micro-structure, porosity, water adsorption and compressive strength were greatly affected by the components’ concentration, in particular the content of silk fibroin. SEM observations showed that the scaffolds of nHA/SF/CTS are highly porous, with pore size in wide range from 25 to 300 µm which is suitable for cell growth. nHA/SF/CTS scaffolds have sufficient mechanical integrity to resist handling during implantation and in vivo loading. Both, the compressive modulus and compressive strength of the scaffold, decrease with the increase in silk fibroin content.

中文翻译：

---

基于丝素蛋白、壳聚糖和纳米羟基磷灰石的新材料的制备与表征

摘要 本文采用冻干法成功制备了一系列多孔纳米羟基磷灰石/丝素蛋白/壳聚糖（nHA/SF/CTS）支架材料。通过衰减全反射傅里叶变换红外光谱、机械测试和热重分析对生物材料进行表征。此外，还进行了孔隙率、孔径、溶胀特性和体外降解试验的研究。研究证明，成分的浓度，特别是丝素蛋白的含量，对微观结构、孔隙率、吸水率和抗压强度影响很大。SEM 观察表明，nHA/SF/CTS 的支架具有高度多孔性，孔径范围从 25 到 300 µm，适合细胞生长。nHA/SF/CTS 支架具有足够的机械完整性，可以在植入和体内加载过程中抵抗处理。支架的压缩模量和压缩强度都随着丝素蛋白含量的增加而降低。